跳转至内容
Merck
CN
所有图片(6)

文件

D8418

Sigma-Aldrich

二甲基亚砜

for molecular biology

别名:

分子生物学级DMSO, 甲基亚砜, 甲基硫酰基甲烷, DMSO

登录查看公司和协议定价
所有图片(6)

About This Item

线性分子式:
(CH3)2SO
CAS号:
67-68-5
分子量:
78.13
Beilstein:
506008
EC 号:
200-664-3
MDL编号:
MFCD00002089
UNSPSC代码:
12191502
PubChem化学物质编号:
24894214
NACRES:
NA.52

等级

for molecular biology

质量水平

200

蒸汽密度

2.7 (vs air)

蒸汽压

0.42 mmHg ( 20 °C)

产品线

BioReagent

检测方案

≥99.9%

形式

liquid

自燃温度

573 °F

保质期

Recommended retest period - 2 years

expl. lim.

42 %, 63 °F

技术

DNA sequencing: suitable
PCR: suitable
transfection: suitable

杂质

≤0.001 meq/g Titratable acid
≤0.1% water (Karl Fischer)

颜色

colorless

折射率

n20/D 1.479 (lit.)

bp

189 °C (lit.)

mp

16-19 °C (lit.)

溶解性

H2O: miscible (completely)

密度

1.10 g/mL (lit.)

吸光度

passes test

适用性

suitable for molecular biology

异质活性

DNase and RNase, none detected

储存温度

room temp

SMILES字符串

CS(C)=O

InChI

1S/C2H6OS/c1-4(2)3/h1-2H3

InChI key

IAZDPXIOMUYVGZ-UHFFFAOYSA-N

正在寻找类似产品? 访问 产品对比指南

相关类别

一般描述

特点及优势：

  • 适于分子应用
  • 无核酸酶DMSO
  • 不含DNase、RNase、蛋白酶和磷酸酶
  • 分子生物学级DMSO

二甲基亚砜（DMSO）是一种对有机和无机化学品具有出色溶剂性质的高极性有机试剂。
该产品为分子生物学级，适用于分子生物学应用，对其中核酸酶和蛋白酶的存在也进行了分析。

应用

DMSO（二甲基亚砜）是一种在有机化学和分子生物学中具有诸多应用的高度极性、非质子有机溶剂。DMSO通常用于

  • 聚合酶链式反应 (PCR)
  • cDNA文库扩增
  • DNA测序
  • poly（A）+ RNA筛选的上柱缓冲液
  • 感受态大肠杆菌转化缓冲液
  • 转染方案。

二甲基亚砜已用于-

  • 用作芯片点样应用中的寡核苷酸溶剂[1]
  • 用作miRNA芯片分析和实时荧光定量PCR (qRT-PCR)中的溶剂
  • 在MTT(3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四唑溴化物）测定的细胞裂解液
  • 制备MMP（线粒体膜电位）细胞荧光评估过程所用的储备溶液
  • 用作储存人类和动物细胞系和噬菌体λ的冷冻保护剂。
制备无菌过滤DMSO溶液时，建议使用PTFE或尼龙膜。不建议使用醋酸纤维素膜。

特点和优势

该产品不含DNase、RNase、磷酸酶和蛋白酶。

注意

在室温下容易过冷并缓慢重融。 固化产品可以通过加热至室温来重新液化，而不会损害产品。

其他说明

2024年CiteAb奖——成功的帕金森研究供应商(2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research)

建议的防溅射手套

产品编号
说明
价格

相关产品

产品编号
说明
价格

通常也和此产品一起购买

产品编号
说明
价格

储存分类代码

10 - Combustible liquids

WGK

WGK 1

闪点(°F)

188.6 °F - closed cup

闪点(°C)

87 °C - closed cup

个人防护装备

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

已有该产品？

在文件库中查找您最近购买产品的文档。

访问文档库

  1. How does the storage temperature relate to shipping conditions?

    The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment. See Shipping and Storage for more information.

  2. What is the molarity of DMSO?

    DMSO will have a molarity of 14.1M, based on a density of 1.1 g/mL and a molecular weight of 78.13 g/mol.

  3. What is the DMSO dissolved in?

    DMSO is not dissolved in any solvent. It is a pure chemical compound that is a liquid at room temperature.

  4. Where does DMSO come from?

    DMSO is known to have been isolated from tree bark in the past. It is now a commericially synthesized solvent.

  5. Does DMSO have any synthetic utility?

    DMSO has been used to oxidize thiols and disulfides to sulfonic acids, hydrolysis of epoxides, thioalkylation of phenols, and oxidation of primary alcohols and halides, and esters of primary alcohols to aldehydes.

  6. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  7. Dimethyl sulfoxide (DMSO) should be a liquid.  Why did it arrive as a solid?

    DMSO supercools easily, and re-melts slowly at room temperature. The melting point is 16-19 °C. The solidified product can be re-liquefied by warming to room temperature without detriment to the product.

  8. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  9. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  10. Is Dimethyl sulfoxide a sterile product?

    No, this product is not considered sterile.  Product Nos. D2560 and D2438 are sterile-filtered.

  11. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  12. What can I use to solubilize Product D8418, Dimethyl sulfoxide?

    This product is miscible in water (1 mL DMSO + 1 mL H2O), yielding a clear, colorless solution. DMSO is a very hygroscopic liquid and should protected from exposure to moisture. DMSO is also miscible with ethanol, acetone, ether, benzene, and chloroform.

  13. Is Product D8418, Dimethyl sulfoxide, stable under extreme conditions?

    DMSO is stable up to 100°C in alkaline, acidic and neutral conditions. At temperatures approaching its boiling point, DMSO is stable in neutral or alkaline conditions.

  14. Is Product D8418, Dimethyl sulfoxide, assayed for DNase, RNase and protease?

    Yes. It is a molecular biology grade product, and it is tested for DNase, RNase and protease. For a lot to pass none of those enzymes may be detected.

  15. Why is Product D8418, Dimethyl sulfoxide, considered as an exceptional organic solvent?

    Dimethyl sulfoxide (DMSO) is a highly polar organic reagent that has exceptional solvent properties for organic and inorganic chemicals.

  16. With what materials is Product D8418, Dimethyl sulfoxide, compatible?

    The compatibility of DMSO with various materials is listed here: Compatible: LDPE, HDPE, polypropylene, PPCO (polypropylene copolymer), polymethylpentene, nylon, PTFE (Teflon) and FEP. Moderately compatible: polystyrene, ECTFE/ETFE. Incompatible: polysulfone, flexible and rigid PVC tubing, polycarbonate.

  17. DMSO, Product D8418 Dimethyl sulfoxide, is used to freeze and store human and animal cell lines. Is there a procedure for it?

    The procedure to freeze cells is in the product information sheet (under Documents, above).

  18. I received Product D8418, Dimethyl sulfoxide, as a frozen solid. But isn't it supposed to be in the liquid state?

    DMSO super cools easily and remelts slowly at room temperature. The product may arrive as a solid instead of a liquid. The solidified product can be reliquified by warming to room temperature without detriment to the product.

  19. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Caroline Godfrey et al.
Human molecular genetics, 24(15), 4225-4237 (2015-05-03)
Splice modulation therapy has shown great clinical promise in Duchenne muscular dystrophy, resulting in the production of dystrophin protein. Despite this, the relationship between restoring dystrophin to established dystrophic muscle and its ability to induce clinically relevant changes in muscle
Claudia C Faria et al.
Cancer research, 75(1), 134-146 (2014-11-14)
Medulloblastoma is the most common malignant pediatric brain tumor, with metastases present at diagnosis conferring a poor prognosis. Mechanisms of dissemination are poorly understood and metastatic lesions are genetically divergent from the matched primary tumor. Effective and less toxic therapies
Phillip McDonagh et al.
Veterinary microbiology, 174(3-4), 438-447 (2014-12-04)
Feline infectious peritonitis (FIP), a feline coronavirus (FCoV) induced disease, is almost invariably fatal with median life expectancy measured in days. Current treatment options are, at best, palliative. The objectives of this study were to evaluate a panel of nineteen
Rory W Blackler et al.
British journal of pharmacology, 172(4), 992-1004 (2014-10-10)
Hydrogen sulphide is an important mediator of gastrointestinal mucosal defence. The use of non-steroidal anti-inflammatory drugs (NSAIDs) is significantly limited by their toxicity in the gastrointestinal tract. Particularly concerning is the lack of effective preventative or curative treatments for NSAID-induced
A Oguro-Ando et al.
Molecular psychiatry, 20(9), 1069-1078 (2014-10-15)
Rare maternally inherited duplications at 15q11-13 are observed in ~1% of individuals with an autism spectrum disorder (ASD), making it among the most common causes of ASD. 15q11-13 comprises a complex region, and as this copy number variation encompasses many
查看所有相关文献

商品

酵母转化简介

转化是将外源DNA引入细胞，引起遗传变异或基因修饰的过程。1928年，Griffith首次报道了利用肺炎链球菌进行的转化。Avery等人于1944年证明了DNA转化的原理。

Yeast Transformation Introduction

Transformation introduces exogenous DNA into cells, a fundamental genetic modification process demonstrated in Streptococcus pneumoniae.

SeqPlex™-I Transcriptome Amplification Kits

SeqPlex™-I WTA kit amplifies RNA for NGS, enabling genomic studies from limited samples.

实验方案

Yeast Transformation Protocols

Yeasts are considered model systems for eukaryotic studies as they exhibit fast growth and have dispersed cells.

酵母转化实验方法

酵母因能够快速生长并具有分散的细胞而被认为是用于真核生物研究的模范系统。

Forskolin-Induced Swelling Assays using Millicell® Microwell Plates

Learn how to cultivate similar-sized iPSC-derived colon organoids using Millicell® Microwell plates and perform a forskolin-induced swelling assay.

Released N-Linked Glycan Analysis Workflow of Adalimumab

A step-by-step protocol for released N-linked glycan analysis of the monoclonal antibody adalimumab, based on UHPLC-FLR-MS and procainamide labeling.

相关内容

KOD One™ PCR Master Mix for Ultra-Fast, High-Fidelity PCR

KOD One™ PCR Master Mix overview for ultra-fast PCR with high specificity, fidelity, and yield

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门