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安全信息

P1107

Sigma-Aldrich

JumpStart™ REDTaq® ReadyMix™ 反应混合物

for High-throughput PCR of complex templates

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别名:
High throughput PCR, Hot start PCR master mix, Hot start master mix, Hot start taq master mix
MDL编号:
MFCD00166026
UNSPSC代码:
41106300
NACRES:
NA.55

质量水平

200

形式

liquid

用途

sufficient for 100 reactions
 sufficient for 400 reactions

特点

Difficult Templates/Specialty Enzymes PCR
dNTPs included
hotstart

浓度

0.75 units/reaction (50 μL reaction volume)

技术

PCR: suitable

颜色

red

输入

purified DNA

应用

agriculture

运输

wet ice

储存温度

−20°C

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一般描述

JumpStart REDTaq ReadyMix PCR Reaction Mix for High Throughput PCR is formulated with REDTaq Genomic DNA polymerase for amplification of more complex templates and genomic templates. This mix contains optimized enzyme and dye concentrations to provide increased length and yield on the more difficult templates.
JumpStart REDTaq ReadyMix PCR反应混合物是制备好的溶液，结合了热启动PCR的性能优势和Sigma ReadyMix和REDTaq的双重便利性。反应混合物优化的2x反应浓缩物中包括Sigma的JumpStart Taq DNA聚合酶、99％纯度的脱氧核苷酸、缓冲液和惰性红色染料。将25 μL ReadyMix加到引物、模板和水中，使最终反应体积为50 μL。在室温下，反应混合物中的JumpStart Taq抗体可使Taq DNA聚合酶失活。在PCR的第一次变性步骤期间，复合物解离，聚合酶活性完全恢复。惰性红色染料不影响自动测序、限制性酶切、连接或其他下游操作。如果需要，只需通过标准纯化方法就可将PCR产物与染料轻松分离。热启动机制允许设置室温，使其成为高通量应用的理想选择 。

应用

JumpStart™REDTaq®ReadyMix™ Reaction Mix has been used:
  • For multiplex PCR
  • For amplification with reduced non-specific amplification
  • For high throughput PCR amplification of complex templates
  • In the amplification of primers developed for the pathogenicity-associated genes of Clavibacter michiganensis
  • For PCR amplification of the human TMPRSS6 gene

特点和优势

  • JumpStart™ REDTaq® DNA聚合酶是一种抗体失活的热启动酶，旨在最大限度地减少非特异性扩增，同时提高目标产量和特异性
  • 无论模板浓度如何，JumpStart Taq DNA聚合酶均可提供优越的扩增效果
  • REDTaq JumpStart ReadyMix可减少移液步骤，降低污染风险。 热启动机制允许设置室温，使其成为高通量应用的理想选择 。
  • 惰性红色染料方便验证试剂是否适当混合
  • 不需要装载其他染料。可以在反应后将等分试样直接加载到琼脂糖凝胶上

包装

默认的反应体积为50 μL

20RXN包装成1 X 500 μL
100RXN包装成1 X 2.5 mL
800RXN包装成1 X 20 mL

单位定义

在74℃下，一个单位可在30分钟内将10 nM总dNTP掺入酸可沉淀的DNA中。

其他说明

想查看JumpStart REDTaq和ReadyMix产品的更多详细信息，请访问 www.sigma-aldrich.com/hotstart。

法律信息

凡购买本产品，即表示授权用户使用产品说明书所指定的专利，但仅限于将所购数量的产品用于内部研究用途。我们未明确表示、暗示，或通过禁止反言形式转让任何其他专利权（如，5′ Nuclease Process专利权）。有关购买许可的更多信息，可联系Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA获取。
依照美国专利号5,338,671和5,587,287和其他地区对应的专利，许可抗体用于体外研究用途。
Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: US 8,404,464 and US 7,972,828. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims.
JumpStart is a trademark of Sigma-Aldrich Co. LLC
REDTaq is a registered trademark of Merck KGaA, Darmstadt, Germany
ReadyMix is a trademark of Sigma-Aldrich Co. LLC

象形图

Exclamation mark
GHS07

警示用语：

Warning

危险声明

H317 - H412

危险分类

Aquatic Chronic 3 - Skin Sens. 1

储存分类代码

10 - Combustible liquids

WGK

WGK 3

法规信息

常规特殊物品
含少量动物源组分生物产品

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Jarred Yasuhara-Bell et al.
International journal of systematic and evolutionary microbiology, 65(Pt 3), 811-826 (2014-12-08)
The genus Clavibacter contains one recognized species, Clavibacter michiganensis. Clavibacter michiganensis is subdivided into subspecies based on host specificity and bacteriological characteristics, with Clavibacter michiganensis subsp. michiganensis causing bacterial canker of tomato. Clavibacter michiganensis subsp. michiganensis is often spread through
E Beutler et al.
Blood cells, molecules & diseases, 44(1), 16-21 (2009-10-13)
Male subjects with iron deficiency from the general population were examined for polymorphisms or sporadic mutations in TMPRSS6 to identify genetic risk factors for iron deficiency anemia. Three uncommon non-synonymous polymorphisms were identified, G228D, R446W, and V795I (allele frequencies 0.0074
John C Hafner et al.
Journal of biogeography, 38(6), 1077-1097 (2011-08-13)
AIM: The rodent genus Microdipodops (kangaroo mice) includes two sand-obligate endemics of the Great Basin Desert: M. megacephalus and M. pallidus. The dark kangaroo mouse, M. megacephalus, is distributed throughout the Great Basin and our principal aims were to formulate
Richard Splivallo et al.
Frontiers in microbiology, 10, 1437-1437 (2019-07-19)
Truffle fungi are well known for their enticing aromas partially emitted by microbes colonizing truffle fruiting bodies. The identity and diversity of these microbes remain poorly investigated, because few studies have determined truffle-associated bacterial communities while considering only a small
Wenqin Wang et al.
BMC plant biology, 10, 205-205 (2010-09-18)
Members of the aquatic monocot family Lemnaceae (commonly called duckweeds) represent the smallest and fastest growing flowering plants. Their highly reduced morphology and infrequent flowering result in a dearth of characters for distinguishing between the nearly 38 species that exhibit
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