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GRSA

Sigma-Aldrich

谷胱甘肽还原酶检测试剂盒

Sufficient for 100 colorimetric tests

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别名:
Glutathione Reductase Activity Assay Kit
UNSPSC代码:
12161503
NACRES:
NA.28

质量水平

200

用途

sufficient for 100 tests

检测方法

colorimetric

储存温度

2-8°C

基因信息

human ... GSR(2936)

相关类别

一般描述

谷胱甘肽还原酶(GR)是一种普遍存在的酶，用于催化氧化型谷胱甘肽(GSSG)还原为谷胱甘肽(GSH)。谷胱甘肽还原酶对于谷胱甘肽氧化还原循环至关重要，可保持足够水平的还原性细胞谷胱甘肽，而后者作为抗氧化剂与游离基和有机过氧化物发生反应。谷胱甘肽也是谷胱甘肽过氧化物酶和谷胱甘肽s转移酶在有机过氧化物解毒和异种生物代谢中的底物。 

应用

谷胱甘肽还原酶测定试剂盒可用于测定谷胱甘肽还原酶活性，进行氧化应激评估，同时还用于研究防污生物杀菌剂对其影响。

生化/生理作用

该试剂盒提供用于分光光度测定的试剂，用于通过跟踪由NADPH 氧化引起的A340的减少或由二硫代双（2-硝基苯甲酸）的减少引起的A412的增加来测量谷胱甘肽还原酶的活性。
谷胱甘肽还原酶(EC 1.6.4.2) (GR)是一种广泛存在的酶，可还原氧化型谷胱甘肽(GSSG)生成谷胱甘肽(GSH)。谷胱甘肽还原酶对谷胱甘肽氧化还原循环至关重要，后者可以维持细胞内充足的还原型GSH水平。GSH是一种抗氧化剂，可以清除自由基和有机过氧化物。 在有机过氧化物解毒过程和外源性物质代谢过程中，谷胱甘肽也是谷胱甘肽过氧化物酶和谷胱甘肽S转移酶的底物。该试剂盒还提供多种分光光度法检测试剂，可通过追踪NADPH氧化引起的340 nm处吸光度降低（紫外分光光度法）或二硫双（2-硝基苯甲酸）(DTNB)还原引起的412 nm处吸光度升高（比色法）来测定谷胱甘肽还原酶的活性。该试剂盒还提供多种分光光度法检测试剂，可通过追踪NADPH氧化引起的A340降低或二硫双（2-硝基苯甲酸）还原引起的A412升高来测定谷胱甘肽还原酶的活性。

适用性

适于检测生物样品中的谷胱甘肽还原酶活性

原理

该试剂盒包含分光光度法测定谷胱甘肽还原酶活性的试剂，即通过跟踪NADPH在340 nm处氧化引起的吸光度下降(紫外测定)或由二硫代双（2-硝基苯甲酸）在412 nm处的还原引起的吸光度增加(比色测定)。该试剂盒提供用于分光光度测定的试剂，即通过跟踪由NADPH氧化引起的A340的减少或由二硫代双（2-硝基苯甲酸）的还原引起的A412的增加。

分析说明

紫外分光光度法检测：在25℃ pH7.5条件下，一单位酶可将1.0 μmol NADPH 氧化。
比色法检测：在25℃ pH7.5条件下，一单位酶可以将1.0 μmol DTNB 还原为 TNB。

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书
  • D81305,5′-Dithiobis(2-nitrobenzoic acid) 50 mg化学品安全说明书
  • G4626Glutathione, oxidized, disodium salt 100 mg化学品安全说明书
  • N6505β-Nicotinamide adenine dinucleotide 25 mg化学品安全说明书

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品
含少量动物源组分生物产品

分析证书(COA)

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  1. How does the storage temperature relate to shipping conditions?

    The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment. See Shipping and Storage for more information.

  2. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  3. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  4. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  5. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  6. In your product bulletin you say you have tested Product GRSA, Glutathione Reductase Assay Kit, on rabbit reticulocyte lysate and Saccharomyces extract. Have you tested it on other species?

    At this time, we have used the kit to test only these species. We have received inquiries concerning its use for rat and higher plant tissue, but we have no data at this time.

  7. Do you know if Product GRSA, Glutathione Reductase Assay Kit, can be used with a 96 well plate reader?

    We have used this kit ony with a UV/Vis spectrophotometer with 1 cm pathlength cuvettes. We have not attempted to modify it for use with a plate reader.

  8. Is Product GRSA, Glutathione Reductase Assay Kit, compatible with your CelLytic cell lysis reagents?

    We have not tried using this kit with our CelLytic reagents. We believe it should be suitable for use with C2978 for mammalian cell lysis or C4882 for yeast cell lysis.

  9. Is Product GRSA, Glutathione Reductase Assay Kit, suitable for use for GR determination in whole blood?

    We do not recommend the kit for use with whole blood due to interference from hemoglobin.

  10. In your brochure for Nitric Oxide and Cell Stress you present absorbance data from yeast lysis. How were the yeast prepared?

    Here is the procedure as recommended by our Production group (March 2008): Grow S. cerevisiae (we used GP460 private strain) on YEPD medium; harvest cells by centrifugation. Wash the cells [7g]  with 20 ml of 20 mM HEPES buffer, pH 7.0. Discard supernatant. Add 1 ml HEPES buffer to make a thick slurry and pour dropwise into liquid nitrogen. Take approx. 2 g of frozen pellet and homogenize with UltraTurrax T-25 while still in liquid nitrogen to make a fine powder. After all the liquid nitrogen has evaporated, take approximately 2 mL of HEPES buffer and add to the 2 g of finely powdered cells. Centrifuge for 5 min in an Eppendorf (benchtop) centrifuge at maximum speed. The supernatant  liquid is the source of the enzyme. The total amount of protein received from this procedure was 21 mg (8.8 mg/mL in 2.4 mL total volume). We determined an activity of approximately 0.3 units/mL, using both the UV and DTNB assays.

  11. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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