推荐产品
生物来源
yeast
表单
suspension
比活
~120 units/mg protein (At 25 °C with oxidized glutathione as the substrate; standardized with BSA.)
包装
pkg of 1 mL (5 mg)
制造商/商品名称
Roche
储存温度
2-8°C
一般描述
Glutathione:NADP+ oxidoreductase
应用
Glutathione reductase (GR) has been used for the reduction of GSSG (glutathione disulfide) to GSH (glutathione).
生化/生理作用
Glutathione Reductase (GR) is a NADPH-dependent flavoprotein that catalyzes the reduction of glutathione disulfide (GSSG) to glutathione (GSH). It is involved in the maintenance of glutathione levels, which is important for oxidation–reduction processes, detoxication and several other cellular functions. In yeast, GR protects the cells from oxidative stress and is transcriptionally regulated by yAP-1 (yes-associated protein 1) protein.
质量
Contaminants: <0.01% NADPH oxidase and 6-PGDH each, <0.1% G6P-DH
外形
Suspension in 3.2 M ammonium sulfate solution, pH approximately 6
制备说明
The ammonium sulfate suspension is stable at 2 to 8 °C until the expiration date printed on the label. It may be used directly in assays for glutathione.
Activator: EDTA prevents the formation of enzyme aggregates. Phosphate stimulates the NADH-dependent reaction.
Activator: EDTA prevents the formation of enzyme aggregates. Phosphate stimulates the NADH-dependent reaction.
其他说明
仅用于生命科学研究。不可用于诊断。
相关产品
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 1
闪点(°F)
does not flash
闪点(°C)
does not flash
法规信息
新产品
C M Grant et al.
Molecular microbiology, 21(1), 171-179 (1996-07-01)
Glutathione (GSH) is an abundant cellular thiol which has been implicated in many cellular processes including protection against xenobiotics, carcinogens and free radicals. Utilization of GSH in both enzymic and non-enzymic defence mechanisms results in its conversion to the oxidized
Glutathione reductase.
I Carlberg et al.
Methods in enzymology, 113, 484-490 (1985-01-01)
Trent E Tipple et al.
Methods in molecular biology (Clifton, N.J.), 889, 315-324 (2012-06-07)
We present two different methods for determining levels of glutathione in complex biological samples and plasma. The DTNB/GR enzyme recycling method is sensitive and requires no specialized equipment. The HPLC method is particularly useful for situations in which sample amounts
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