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Merck
CN

38185

Sigma-Aldrich

用于氧化和还原性谷胱甘肽的定量试剂盒

200 tests

别名:

γ-L-谷酰基-L-半胱氨酰甘氨酸试剂盒, 定量试剂盒

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About This Item

UNSPSC代码:
12352108
NACRES:
NA.32

质量水平

质量

200 tests

储存温度

2-8°C

相关类别

一般描述

谷胱甘肽定量试剂盒用于检测氧化和还原性谷胱甘肽。 谷胱甘肽通常以还原性形式表示,称为谷胱甘肽;然而,GSH在氧化应激时转变为氧化态(GSSG)。GSH和GSSG的比值被认为是氧化应激的指标。 谷胱甘肽,又称γ-L-谷酰基-L-半胱氨酰甘氨酸,是一种参与抗氧化和药物代谢的三肽化合物。它是谷胱甘肽过氧化物酶、谷胱甘肽s转移酶和硫醇转移酶等酶的底物。

应用

谷胱甘肽定量试剂盒用于检测谷胱甘肽总量,作为氧化应激或疾病风险的指标。

特点和优势

试剂盒检测总谷胱甘肽和GSSG的范围分别为0.5 μmol/l至50 μmol/l和0.5 μmol/l至25 μmol/l。

组分

谷胱甘肽定量试剂盒包含:
  • 酶液(50µl X 1)
  • 缓冲液(60ml X 1)
  • 标准GSH (X 1)
  • 掩蔽剂 (20µl X 1)
  • 辅酶 (X 2)
  • 底物(DTNB) (X4)
  • 标准GSSG (X 1)

分析说明

谷胱甘肽定量试剂盒包含谷胱甘肽掩蔽剂。通过测定DTNB(’5,5-二硫代(2-硝基苯甲酸))与酶循环系统的比色反应获得的吸收(λmax = 412 nm),从而实现了样品中的GSSG检测。 谷胱甘肽的总量可由谷胱甘肽的总量减去谷胱甘肽的总量来测定。

其他说明

未在韩国、新加坡、台湾和日本供应

象形图

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警示用语:

Danger

危险分类

Eye Irrit. 2 - Flam. Liq. 2 - Skin Irrit. 2 - Skin Sens. 1

WGK

WGK 3

法规信息

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