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Merck
CN

G9768

Sigma-Aldrich

鸟苷 5′--三磷酸盐-琼脂糖

aqueous glycerol suspension

别名:

5′-GTP agarose

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About This Item

MDL编号:
UNSPSC代码:
41106500
NACRES:
NA.56

生物来源

bacterial (CORYNEBACTERIUM SP)
enzyme from Porcine brain
plant (Sea weed)
yeast

质量水平

形式

aqueous glycerol suspension

标记范围

1-5 μmol per mL

基质

cross-linked 4% beaded agarose

基质活化

cyanogen bromide

基质附着

ribose hydroxyls

基质隔离区

11 atoms (adipic acid dihydrazide)

储存温度

−20°C

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相关类别

应用

鸟苷 5′-三磷酸-琼脂糖(5′-GTP 琼脂糖)是甘油水溶液悬浮液中的琼脂糖结合物,用于亲和层析和纯化鸟苷结合蛋白

外形

悬浮于含有0.25 M NaCl的50%甘油中
鸟苷5′-三磷酸-琼脂糖已被用于:
  • 用于纯化易位刺激因子(translocation stimulating factor)的色谱柱
  • 在GTP-琼脂糖拉下实验(pull down assay)中测量与GTP结合的RHEB(脑Ras同源蛋白,Ras homolog enriched in brain)的水平
  • GTP结合测定中
  • 消耗大鼠肝细胞溶质中的GTP结合蛋白

WGK

WGK 3


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Reiaz Ul Rehman et al.
Plant & cell physiology, 49(5), 751-766 (2008-04-04)
The regulatory functions of Rab proteins in membrane trafficking lie in their ability to perform as molecular switches that oscillate between a GTP- and a GDP-bound conformation. The role of tomato LeRab11a in secretion was analyzed in tobacco protoplasts. Green
Arsenite stress down-regulates phosphorylation and 14-3-3 binding of leucine-rich repeat kinase 2 (LRRK2), promoting self-association and cellular redistribution
Mamais A, et al.
The Journal of Biological Chemistry, 289, 21386-21400 (2014)
P E Glaser et al.
Biochemistry, 34(38), 12193-12203 (1995-09-26)
Recently we demonstrated that the unique stereoelectronic relationships inherent in the structure of plasmenylethanolamine facilitate membrane fusion, and we postulated the existence of a membrane fusion protein which could exploit the propensity of plasmenylethanolamine molecular species to adapt an inverted
Possible role of both the alpha and beta gamma subunits of the heterotrimeric G protein, Gs, in transcytosis of the polymeric immunoglobulin receptor
Bomsel M and Mostov KE
The Journal of Biological Chemistry, 268, 25824-25835 (1993)
T E Allen et al.
Molecular and biochemical parasitology, 65(2), 233-245 (1994-06-01)
The hypoxanthine-guanine phosphoribosyltransferase (HGPRT) enzyme in Trypanosoma cruzi is a rational target for the treatment of Chagas disease. To evaluate the T. cruzi HGPRT in detail, the HGPRT gene (hgprt) was cloned from a genomic library of T. cruzi DNA

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