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安全信息

F6765

Sigma-Aldrich

胎牛血清

USA origin, Charcoal Stripped, sterile-filtered, suitable for cell culture

别名:

FBS, FCS, 血清

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About This Item

MDL编号:
UNSPSC代码:
12352207
NACRES:
NA.71

生物来源

bovine fetus

质量水平

无菌性

sterile-filtered

产品线

FBS Select

组成

Bovine IgG, ≤1 mg/mL
Hemoglobin, ≤20 mg/dL

来源

USA origin

技术

cell culture | mammalian: suitable

杂质

≤10 EU/mL endotoxin (typically ≤1 EU/ml)

运输

dry ice

储存温度

−20°C

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应用

来自美国的FBS被广泛用于细胞培养应用中。 FBS可为培养的细胞提供了多种未确定的生长促进和存活增强因子。 炭剥离胎牛血清常被用于替代胎牛血清,以最大限度地降低 10% 血清补充培养基中提供的雄激素和其他激素水平。

生化/生理作用

炭剥离胎牛血清(FBS)适用于在研究中需要降低各种激素水平的研究人员。 葡聚糖处理过的木炭用于选择性去除激素,而不会造成其他血清成分非特异性损失。 根据治疗前后 T3、T4 和黄体酮水平的降低评估激素去除的有效性。

该产品专为参与研究受类固醇激素(例如雌激素刺激)影响的过程和肥胖过程,以及其他受益于激素水平降低的领域(例如某些类型的病毒感染)的研究人员设计。

分析说明

  • 内毒素和血红蛋白测试
  • 检测了是否存在细菌、病毒和支原体
  • 在无菌条件下用0.1微米膜过滤三次

其他说明

该产品未在澳大利亚上市。请在这里查看我们消耗激素的碳剥离FBS方案。

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves

法规信息

监管及禁止进口产品

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. Once a bottle of Fetal Bovine Serum (FBS) is thawed, for how long can the bottle be used?

    Once Fetal Bovine Serum has been thawed, the bottle can be used in cell culture for up to 7 days for most cell types when stored  refrigerated at 2-8°C.  If the cells are not fastidous (e.g. HeLa or fibroblasts), the thawed and refrigerated serum can be used for up to 30 days.  For some extremely fastidious cell lines, it may be necessary to screen individual lots for acceptable performance.  For uses such as blocking for various immuno procedures, usage will generally be for 30 days and perhaps somewhat longer when stored at 2-8°C.  For other procedures, usage beyond 30 days storage at 2-8°C will need to be determined.

  6. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Cyril Corbet et al.
Nature communications, 11(1), 454-454 (2020-01-25)
Acidosis, a common characteristic of the tumor microenvironment, is associated with alterations in metabolic preferences of cancer cells and progression of the disease. Here we identify the TGF-β2 isoform at the interface between these observations. We document that acidic pH promotes
Andras Franko et al.
Genes, 11(10) (2020-10-11)
Prostate cancer (PCa), the most incident cancer in men, is tightly regulated by endocrine signals. A number of different PCa cell lines are commonly used for in vitro experiments, but these are of diverse origin, and have very different cell-proliferation
Jonathan T Lei et al.
Cell reports, 24(6), 1434-1444 (2018-08-09)
RNA sequencing (RNA-seq) detects estrogen receptor alpha gene (ESR1) fusion transcripts in estrogen receptor-positive (ER+) breast cancer, but their role in disease pathogenesis remains unclear. We examined multiple ESR1 fusions and found that two, both identified in advanced endocrine treatment-resistant
Christoph Lammel et al.
Biomolecules, 10(9) (2020-08-23)
Twenty natural remedies traditionally used against different inflammatory diseases were probed for their potential to suppress the expression of the inflammatory markers E-selectin and VCAM-1 in a model system of IL-1 stimulated human umbilical vein endothelial cells (HUVEC). One third
Ana M Reyes-Ramos et al.
Cancers, 11(10) (2019-10-30)
The paracrine interaction between tumor cells and adjacent stroma has been associated with the oncogenic activity of the Hedgehog (Hh) pathway in triple-negative breast tumors. The present study developed a model of paracrine Hh signaling and examined the impact of

商品

Review the basics of using fetal bovine (or calf) serum for cell culture and how sourcing and regulations affect the price of sera for cell-based assays.

回顾使用胎牛(或小牛)血清进行细胞培养的基础知识,以及采购和法规如何影响细胞测定所用血清的价格。

Find answers to frequently asked questions (FAQs) about FBS and other serum products, and links to related products.

查找有关FBS和其他血清产品的常见问题解答(FAQ)以及相关产品的链接。

实验方案

Protect your investment with best practices for storing and thawing FBS and other sera. Sample FBS for validation in your cell culture protocol.

相关内容

Explore our portfolio of fetal bovine serum (FBS) and determine the right FBS for your lab needs and budget. Request a serum sample.

Our broad range of the most trusted tools for cell culture includes stringently sourced and tested FBS, established media formulations, and sterile labware. Cutting-edge techniques using stem cells and 3D matrices are enabled by organoids, hydrogels, culture scaffolds, and bioinks for 3D bioprinting.

我们的胎牛血清(FBS)系列可供您寻找适合实验室需求和预算的FBS。索取血清样品。

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

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