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Merck
CN

B4554

BES

BioPerformance Certified, suitable for cell culture, ≥99.0%

Synonym(s):

N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid, N,N-Bis(2-hydroxyethyl)taurine

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About This Item

Empirical Formula (Hill Notation):
C6H15NO5S
CAS Number:
10191-18-1
Molecular Weight:
213.25
UNSPSC Code:
12161700
NACRES:
NA.25
PubChem Substance ID:
24891788
EC Number:
233-465-5
Beilstein/REAXYS Number:
1781572
MDL number:
MFCD00007533

Key Documents

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Product Name

BES, BioPerformance Certified, suitable for cell culture, ≥99.0%

assay

≥99.0%

InChI key

AJTVSSFTXWNIRG-UHFFFAOYSA-N

InChI

1S/C6H15NO5S/c8-4-1-7(2-5-9)3-6-13(10,11)12/h8-9H,1-6H2,(H,10,11,12)

SMILES string

OCCN(CCO)CCS(O)(=O)=O

grade

BioPerformance Certified

form

crystalline

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin and bioburden, tested

pH

2.5-5.0 (25 °C, 213.3 g/L)

useful pH range

6.4-7.8

pKa (25 °C)

7.1

solubility

water: 0.5 g/mL, clear, colorless

application(s)

diagnostic assay manufacturing

foreign activity

DNAse (exonuclease), NICKase (endonuclease), RNAse, and protease, none detected

storage temp.

room temp

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Application

BES buffer has been used:

  • as a supplement in opti-minimal essential medium (MEM) culture media to culture thymocytes for In vitro analysis of thymocyte apoptosis
  • as a component of borate buffer saline (BBS) for adeno-associated virus (AAV) vector production, extraction, and purification
  • as a component of diluent one for semen collection and cryopreservation

General description

N-N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid (BES) is a zwitterionic sodium sulphate buffer used in biochemistry and molecular biology research. BES buffer used as a secondary pH standard can help maintain a stable pH range in solutions, particularly in the range of 6.2-7.6 pH range of 6.6 to 7.4. It ​is used in a study to evaluate that buffers minimize the action of interfering factors in the bacterial endotoxins test. The application of BES as a buffer solution for calcium phosphate-mediated transfection of eukaryotic cells with plasmid DNA has also been studied.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
SLCJ1316
SLCG2320
SLBS5966
SLBN9753V
015K54151

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Standard buffer of N, N-bis (2-hydroxyethyl)-2-aminoethanesulfonic acid (Bes) for use in the physiological pH range 6.6 to 7.4
Roy R N, et al.
Analytical Chemistry, 47(8) (1975)
Cryopreservation of Korean Oge chicken semen using N-methylacetamide
Hong Jo L, et al.
Cryo letters, 33(6), 427-434 (2012)
Effects of amino acid sequence, buffers, and ionic strength on the rate and mechanism of deamidation of asparagine residues in small peptides
Tyler-Cross R and Schirch V
The Journal of Biological Chemistry, 266(33) , 22549-22556 (1991)
Calcium-phosphate-mediated Transfection of Eukaryotic Cells with Plasmid DNAs
Sambrook J and Russell D W
CSH Protocols, 2006(1) (2006)
Saline and buffers minimize the action of interfering factors in the bacterial endotoxins test
Fujita Y, et al.
Analytical Biochemistry, 409(1), 46-53 (2011)
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