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391334

Millipore

BES

ULTROL® Grade, ≥99% dry basis (titration), zwitterionic N-substituted aminosulfonic acid buffer

Synonym(s):

BES, Free Acid, ULTROL® Grade, N,N- bis-(2-Hydroxyethyl)-2-aminoethanesulfonic Acid

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About This Item

Empirical Formula (Hill Notation):
C6H15NO5S
CAS Number:
10191-18-1
Molecular Weight:
213.25
MDL number:
MFCD00007533
UNSPSC Code:
12161700
NACRES:
NA.25

Quality Level

100

Assay

≥99% dry basis (titration)

form

solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

color

white

pKa (20 °C)

7.12

solubility

water: 1 mg/mL

cation traces

heavy metals: ≤1 ppm

shipped in

ambient

storage temp.

15-25°C

InChI

1S/C6H15NO5S/c8-4-1-7(2-5-9)3-6-13(10,11)12/h8-9H,1-6H2,(H,10,11,12)

InChI key

AJTVSSFTXWNIRG-UHFFFAOYSA-N

General description

Absorbance (100 mM, 260 nm):≤0.05.
BES buffer is suitable for use in systems where a calcium phosphate-DNA complex formation is desirable (Absorbance: <0.05 at 260 nm, 100 mM). Chen and Okayama describe an improved transfection system for the stable transformation of cells with plasmid DNA using BES buffer. The slow formation appears to be the most critical factor contributing to the high efficiency of stable transformations. Has a pKa of 7.12 at 20°C.
BES buffer is suitable for use in systems where a calcium phosphate-DNA complex formation is desirable. Chen and Okayama described an improved transfection system for the stable transformation of cells with plasmid DNA using BES buffer. The gradual formation of the calcium phosphate-DNA complex and its slow precipitation onto cells appears to be the most critical factor contributing to the high efficiency of stable transformations.

Warning

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 2 weeks at 4°C.

Other Notes

De Graeve, K.G., et al. 1994. J. Appl. Bacteriol. 76, 55.
Chen, C.A., and Okayama, H. 1988. Biotechniques6, 632.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTROL is a registered trademark of Merck KGaA, Darmstadt, Germany

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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