391334
BES
ULTROL® Grade, ≥99% dry basis (titration), zwitterionic N-substituted aminosulfonic acid buffer
Synonym(s):
BES, Free Acid, ULTROL® Grade, N,N- bis-(2-Hydroxyethyl)-2-aminoethanesulfonic Acid
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About This Item
Empirical Formula (Hill Notation):
C6H15NO5S
CAS Number:
Molecular Weight:
213.25
MDL number:
UNSPSC Code:
12161700
NACRES:
NA.25
Pricing and availability is not currently available.
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Quality Level
Assay
≥99% dry basis (titration)
form
solid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
color
white
pKa (20 °C)
7.12
solubility
water: 1 mg/mL
cation traces
heavy metals: ≤1 ppm
shipped in
ambient
General description
Absorbance (100 mM, 260 nm):≤0.05.
BES buffer is suitable for use in systems where a calcium phosphate-DNA complex formation is desirable (Absorbance: <0.05 at 260 nm, 100 mM). Chen and Okayama describe an improved transfection system for the stable transformation of cells with plasmid DNA using BES buffer. The slow formation appears to be the most critical factor contributing to the high efficiency of stable transformations. Has a pKa of 7.12 at 20°C.
Warning
Toxicity: Standard Handling (A)
Reconstitution
Following reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 2 weeks at 4°C.
Other Notes
De Graeve, K.G., et al. 1994. J. Appl. Bacteriol. 76, 55.
Chen, C.A., and Okayama, H. 1988. Biotechniques6, 632.
Chen, C.A., and Okayama, H. 1988. Biotechniques6, 632.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTROL is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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