All Photos(5)
Synonym(s):
2-(bis(2-hydroxyethyl)amino)ethane sulfonic acid, n,n-bis[2-hydroxyethyl]-2-aminoethanesulfonic acid, N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid, N,N-Bis(2-hydroxyethyl)taurine
Empirical Formula (Hill Notation):
C6H15NO5S
CAS Number:
Molecular Weight:
213.25
Beilstein:
1781572
EC Number:
MDL number:
UNSPSC Code:
12161700
PubChem Substance ID:
NACRES:
NA.25
Recommended Products
Quality Level
Assay
≥99.0% (titration)
form
crystalline
storage condition
dry at room temperature
color
white
pH
2.5-5.0 (25 °C, 213.3 g/L)
useful pH range
6.4-7.8
pKa (25 °C)
7.1
solubility
water: 0.5 g/mL, clear, colorless
density
1.30 g/cm3 at 20—25 °C
1.35 g/cm3 at 20—25 °C
application(s)
clinical research
diagnostic assay manufacturing
general analytical
life science and biopharma
storage temp.
room temp
SMILES string
OCCN(CCO)CCS(O)(=O)=O
InChI
1S/C6H15NO5S/c8-4-1-7(2-5-9)3-6-13(10,11)12/h8-9H,1-6H2,(H,10,11,12)
InChI key
AJTVSSFTXWNIRG-UHFFFAOYSA-N
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General description
N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid, known as BES, is a zwitterionic buffer and a valuable secondary standard in biochemical research. BES, a sulfonic acid-containing cross-linking agent, induces cross-linking between sulfonated polyimide chains in sulfonated polyimide membranes. The useful pH range for BES buffer is 6.4 to 7.8. It is one of the "Good" buffers developed in the 1960s to provide buffering capacity in the pH range of 6.15 to 8.35, making it widely applicable in biochemical studies. BES buffer is suitable for use in systems where the formation of a calcium phosphate-DNA complex is desirable (Absorbance: < 0.05 at 260 nm, 100 mM). Further, BES buffer can be employed in the study of molecular biology, biological buffers, molecular biology reagents, and zwitterionic compounds.
Application
BES may be employed as binding buffer in modified Eagle′s medium during the binding assay of human melanoma cells. It may be used as biobuffer to investigate the aqueous medium self-assembly of heterometallic CuII/Li 3D coordination polymers.
Further, BES has found application in transfection studies, both in the preparation of reagents for transfection and in signaling assays related to Neural Melanocortin Receptors.
Features and Benefits
- Suitable for Cell Culture media and as a Buffering solution in Biochemical and Molecular Biology Research
- Effective Buffering from pH 2.5-5.0 (25 °C, 213.3 g/L) with a pKa of 7.1 (25 °C)
- Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
Other Notes
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comparable product
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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Second-stage dissociation of N,N-bis (2-hydroxyethyl)-2-aminoethanesulfonic acid (?bes?) in water and in 50 mass per cent methanol+ water from 278.15 to 328.15 K.
Roy RN, et al.
The Journal of Chemical Thermodynamics, 9(4), 325-332 (1977)
Water sorption, proton conduction, and methanol permeation properties of sulfonated polyimide membranes cross-linked with N, N-bis (2-hydroxyethyl)-2-aminoethanesulfonic acid (BES).
Lee CH, et al.
Macromolecules, 39(2), 755-764 (2006)
Marina V Kirillova et al.
Inorganic chemistry, 51(9), 5224-5234 (2012-04-10)
The facile aqueous medium reactions of copper(II) nitrate with BES biobuffer [(HOCH(2)CH(2))(2)N(CH(2)CH(2)SO(3)H), hereinafter referred as H(3)bes] in the presence of various benzenecarboxylic acids [benzoic (Hba), 3-hydroxybenzoic (Hhba), and 3,5-dihydroxybenzoic (Hdhba) acid] and lithium hydroxide gave rise to the self-assembly generation
R N Fabricant et al.
Proceedings of the National Academy of Sciences of the United States of America, 74(2), 565-569 (1977-02-01)
Purified mouse nerve growth factor (NGF)radiolabeled with 125I was tested for its ability to bind to a variety of different cultured cells. NGF binds readily to human and hamster melanoma cells but does not bind to many other cell lines.
Logan Y Brown et al.
STAR protocols, 1(3), 100152-100152 (2020-12-31)
Lentiviral vectors are an ideal gene-delivery system for large gene-editing tools, such as the clustered regularly interspaced short palindromic repeat (CRISPR)-Cas9 system, due to their high packaging capacity and broad tropism. Here, we present a calcium phosphate-based protocol for lentiviral
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