一般描述
酰基辅酶 A 合成酶属于腺苷酸合成酶超家族。具有保守的三磷酸腺苷/单磷酸腺苷 (ATP/AMP) 结合基序。
应用
来自假单胞菌属的酰基辅酶 A 合成酶，已：
- 用作连接酶合成三磷酸腺苷 (ATP) 的甲羟戊酸衍生物
- 在 体外 脂肪酰化试验
- 合成 ( 14 C) 维诺辅酶 a (Va-CoA)，(3) ATP 的二磷酸盐衍生物 (4) 和 ( 3 H) 棕榈酸酯-CoA
酰基辅酶 A 合成酶可用于研究脂肪酸代谢和脂质代谢。已用于研究其与脂肪酸转运蛋白的相互作用，发现其参与脂肪细胞对长链脂肪酸的高效摄取 。
生化/生理作用
酰基辅酶 A (CoA) 合成酶 (ACS) 酶催化两步硫酯化反应，涉及游离脂肪酸 (FAs) 转化为 CoA 酯。ACS 的底物从 2 个碳至 26 个碳不等。参与脂类代谢 FAs 的激活，并使 FA 能够参与各种细胞代谢途径。
酰基辅酶 A 合成酶蛋白参与调节和促进哺乳动物细胞中长链脂肪酸的转运 。
单位定义
1 个单位将从 ATP 生成 1.0 μmol AMP 和油酰基辅酶 A，并在存在辅酶 A 的情况下在 pH 8.1 和 25 ℃ 下形成油酸盐。
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
Teresa J Stradomska
Postepy biochemii, 57(2), 183-190 (2011-09-15)
Peroxisomes, classical compartments of eucaryotic cells have significant functions in cellular metabolism, which beta-oxidation fatty acids and detoxification of H2O2 are the most important biochemical process. Defects in genes encoding for peroxisomal proteins result in biochemical malfunctioning of these organelles
Michael Veit et al.
Methods in molecular biology (Clifton, N.J.), 446, 163-182 (2008-04-01)
Palmitoylation or S-acylation is the post-translational attachment of fatty acids to cysteine residues and is common among integral and peripheral mem brane proteins. Palmitoylated proteins have been found in every eukaryotic cell type examined (yeast, insect, and vertebrate cells), as
A Ruść et al.
Meat science, 89(4), 440-443 (2011-06-10)
The aim of the study was to check whether different genotypes at acyl-CoA synthetase (ACSL4 locus, SNP G2645A) are associated with pork quality. 132 (Landrace × Yorkshire) × Duroc fatteners were genotyped by originally developed PCR-RFLP method. Upon the slaughter
Mariana Cooke et al.
Journal of lipid research, 52(11), 1936-1948 (2011-09-10)
Acyl-CoA synthetase 4 (ACSL4) is implicated in fatty acid metabolism with marked preference for arachidonic acid (AA). ACSL4 plays crucial roles in physiological functions such as steroid synthesis and in pathological processes such as tumorigenesis. However, factors regulating ACSL4 mRNA
M Rachel Richards et al.
Journal of lipid research, 47(3), 665-672 (2005-12-17)
The fatty acid transport proteins (FATP) and long-chain acyl coenzyme A synthetase (ACSL) proteins have been shown to play a role in facilitating long-chain fatty acid (LCFA) transport in mammalian cells under physiologic conditions. The involvement of both FATP and
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