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应用
BCECF 的细胞渗透性酯，通过胞质酯酶水解，产生胞内捕获的 pH 指示剂 BCECF。允许同时记录单个骨肉瘤细胞的细胞体积变化和细胞内 pH 值。
一种不带电的、无荧光的分子，对细胞悬液的非侵入性批量加载有帮助，因为它能透过细胞膜。细胞非特异性酯酶裂解其亲脂性阻断基团，产生带电荷的分子荧光形式。与母体化合物相比，这种带电荷的衍生物以慢得多的速率漏出电池。用于测定灌注组织、细胞间隙 、哺乳动物细胞 、植物细胞、细菌和酵母菌的 pH 值。此外，还用于研究细胞功能属性试验，如粘附、多药耐药性、活力和细胞毒性、细胞凋亡和趋化性。
包装
无底玻璃瓶。内含物在插入的融合锥体内。
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
H W van Veen et al.
The Journal of biological chemistry, 269(47), 29509-29514 (1994-11-25)
The strictly aerobic, polyphosphate-accumulating Acinetobacter johnsonii strain 210A degrades its polyphosphate when oxidative phosphorylation is impaired. The endproducts of this degradation, divalent metal ions and inorganic phosphate, are excreted as a neutral metal-phosphate (MeHPO4) chelate via the electrogenic MeHPO4/H+ symport
R S Haworth et al.
Biochimica et biophysica acta, 1098(1), 79-89 (1991-12-13)
We have tested the efficacy of fluorescent probes for the measurement of intracellular pH in Saccharomyces cerevisiae. Of the compounds tested (fluorescein, carboxyseminaphthorhodafluor-1 (C.SNARF-1) and 2',7'bis(carboxyethyl)-5(6')-carboxyfluorescein), C.SNARF-1 was found to be the most useful indicator of internal pH. Fluorescence microscopy
P J Harris et al.
The American journal of physiology, 266(1 Pt 1), C73-C80 (1994-01-01)
The lateral intercellular spaces (LIS) of reabsorptive epithelia are the site of the proposed local osmotic gradient responsible for transepithelial transport. We developed techniques for loading the LIS of living cultured renal cells (MDCK and LLC-PK1) with the fluorescent dye
M T Alonso et al.
The Biochemical journal, 272(2), 435-443 (1990-12-01)
The effects of arachidonic acid and thrombin on calcium movements have been studied in fura-2-loaded platelets by a procedure which allows simultaneous monitoring of the uptake of manganese, a calcium surrogate for Ca2+ channels, and the release of Ca2+ from
D Molenaar et al.
Biochimica et biophysica acta, 1115(1), 75-83 (1991-11-14)
The cytoplasmic pH of Lactococcus lactis was studied with the fluorescent pH indicator 2',7'-bis-(2-carboxyethyl)-5 (and-6)-carboxyfluorescein (BCECF). A novel method was applied for loading bacterial cells with BCECF, which consists of briefly treating a dense cell suspension with acid in the
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荧光寿命测量与基于强度的测量相比是有优势的。其应用包括荧光寿命分析，感测和FLI。
Fluorescence lifetime measurement is advantageous over intensity-based measurements. Applications include fluorescence lifetime assays, sensing and FLI.
一氧化氮 (NO) 是神经元、内皮细胞和免疫系统中的信号转运分子。
Nitric oxide (NO) as a signal transporter in neurons, endothelial cells and in the immune system.
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