216254
BCECF细胞渗透性酯混合物
A variable mixture of cell-permeable ester derivatives of BCECF that are hydrolyzed by cytosolic esterases to yield intracellularly trapped indicator BCECF.
别名:
BCECF细胞渗透性酯混合物, 2ʹ,7ʹ-双-（羧乙基）-5（6ʹ）-羧基荧光素乙酰氧基甲酯
产品名称
BCECF细胞渗透性酯混合物, A variable mixture of cell-permeable ester derivatives of BCECF that are hydrolyzed by cytosolic esterases to yield intracellularly trapped indicator BCECF.
SMILES string
O1C3(c4c(cc(c(c4)CCC(=O)OCOC(=O)C)OCOC(=O)C)Oc5c3cc(c(c5)OCOC(=O)C)CCC(=O)OCOC(=O)C)c2c(cc(cc2)C(=O)OCOC(=O)C)C1=O
InChI key
NTECHUXHORNEGZ-UHFFFAOYSA-N
assay
≥85% (mixture of isomers, HPLC)
form
solid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
protect from light
color
white to yellow
solubility
acetonitrile: 25 mg/mL
DMF: soluble
DMSO: soluble
fluorescence
λex 500-504 nm
λem 525-531 nm
shipped in
ambient
storage temp.
−20°C
Quality Level
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Biochem/physiol Actions
主要靶标
监测细胞内pH值的变化
监测细胞内pH值的变化
产物不与ATP竞争。
可逆：否
细胞可渗透性：是
Disclaimer
毒性：刺激性（B）
General description
BCECF（目录编号216252）的细胞可渗透酯衍生物的可变混合物，其被胞质酯酶水解以产生细胞内捕获的指示剂BCECF。已用于监测哺乳动物成纤维细胞，胃细胞，淋巴细胞，肌细胞和远曲小管中细胞内pH值的变化。典型的上样缓冲液在不含氨基酸的无血清培养基中包含1-10 µM BCECF/AM，并含有伯胺或仲胺的缓冲液。
Other Notes
Futsaetner, C.M., et al. 1993.Can.J. Microbiol.39, 180.
Hayashi, H., et al. 1992.Am. J. Physiol.262, C628.
Sasaki, S., et al. 1992.Biochim.Biophys.Acta 1137, 45.
Miyata, H., et al. 1989.Biochem.Biophys.Res. Commun.163, 500.
Weiner, I.D., and Hamm, L.L.1989.Am. J. Physiol.256, F957.
Hayashi, H., et al. 1992.Am. J. Physiol.262, C628.
Sasaki, S., et al. 1992.Biochim.Biophys.Acta 1137, 45.
Miyata, H., et al. 1989.Biochem.Biophys.Res. Commun.163, 500.
Weiner, I.D., and Hamm, L.L.1989.Am. J. Physiol.256, F957.
Packaging
用惰性气体包装
Preparation Note
复溶后，等分并冷冻保存（-20°C）。储备液在-20°C下可稳定保存1周。
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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