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MAB4401

Sigma-Aldrich

Anti-Oct-4 Antibody, clone 10H11.2

clone 10H11.2, Chemicon®, from mouse

别名:

Octamer-binding transcription factor 3, POU class 5 homeobox 1, POU domain class 5, transcription factor 1, POU domain, class 5, transcription factor 1, POU-type homeodomain-containing DNA-binding protein, octamer-binding transcription factor-3

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About This Item

UNSPSC代码:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物来源

mouse

抗体形式

purified antibody

克隆

10H11.2, monoclonal

种属反应性

human

制造商/商品名称

Chemicon®

技术

ELISA: suitable
flow cytometry: suitable
immunocytochemistry: suitable
western blot: suitable

输入

sample type induced pluripotent stem cell(s)
sample type: human embryonic stem cell(s)

同位素/亚型

IgG1

NCBI登记号

NM_002701.4

UniProt登记号

Q01860

运输

wet ice

靶向翻译后修饰

unmodified

基因信息

human ... POU5F1(5460)

一般描述

Octamer-4 (Oct-4), a member of the POU family of transcription factors, has been demonstrated to be vital for the formation of self-renewing pluripotent stem cells. During embryogenesis expression of Oct-4 is limited to pluripotent cells of the inner cell mass (ICM) that contribute to the formation of all fetal cell types. This relationship between Oct-4 and pluripotency has seen this transcription factor emerge as a marker of pluripotent stem cells. Undifferentiated human and murine pluripotent Embryonic Stem (ES) and Embryonic Carcinoma (EC) cells express Oct-4. Additionally, murine Embryonic Germ (EG) cells are also known to express Oct-4. Following stem cell differentiation, the level of Oct-4 expression decreases. Oct-4 expression by human EG cells is not known.
Purified monoclonal antibody for the detection of Human Oct-4.

特异性

Reacts with human Oct-4 only, and detects the Oct-4A isoform. (Note: Please order Catalog No. MAB4419 for use with mouse embryonic stem cells).
The clone reacts strongly with specific nuclear binding to human ES cells and cross-reacts weakly with mouse ES cells.

应用

Detect Oct-4 using this Anti-Oct-4 Antibody, clone 10H11.2 validated for use in WB, FC, IC, ELISA.
Flow Cytometry: A previous lot of this antibody was used in FC.

ELISA: A previous lot of this antibody was used in ELISA.

Immunocytochemistry: A previous lot of this antibody was used in IC. H9 cells in culture on a mouse embronic fibroblast feeder layer were labeled by a standard indirect IF protocol. Oct-4 labelling in green, DAPI in blue, & SSEA-3 (MAB4303 in red).

Optimal working dilutions must be determined by the end user.

质量

Evaluated by western blot on mouse P1 brain lysates.

Western Blotting: 1:500 dilution of this antibody detected Oct-4 on 10 µg of mouse P1 brain lysates

目标描述

44 kDa

外形

Format: Purified

分析说明

Control
Human embryonic stem cell lysate.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

分析证书(COA)

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Microporous membrane growth substrates for embryonic stem cell culture and differentiation.
Steven D Sheridan,Sonia Gil,Matthew Wilgo,Aldo Pitt
Methods in Cell Biology null
Efficient derivation of embryonic stem cells from nuclear transfer and parthenogenetic embryos derived from cryopreserved oocytes.
Sung LY, Chang CC, Amano T, Lin CJ, Amano M, Treaster SB, Xu J, Chang WF, Nagy ZP, Yang X, Tian XC
Cellular Reprogramming null
Lingjun Rao et al.
Nature communications, 9(1), 126-126 (2018-01-11)
The generation of functional skeletal muscle tissues from human pluripotent stem cells (hPSCs) has not been reported. Here, we derive induced myogenic progenitor cells (iMPCs) via transient overexpression of Pax7 in paraxial mesoderm cells differentiated from hPSCs. In 2D culture
Chen Liu et al.
PloS one, 9(9), e108239-e108239 (2014-09-27)
Annulus fibrosus (AF) injuries can lead to substantial deterioration of intervertebral disc (IVD) which characterizes degenerative disc disease (DDD). However, treatments for AF repair/regeneration remain challenging due to the intrinsic heterogeneity of AF tissue at cellular, biochemical, and biomechanical levels.
Targeting cancer stem cells through L1CAM suppresses glioma growth.
Bao, S; Wu, Q; Li, Z; Sathornsumetee, S; Wang, H; McLendon, RE; Hjelmeland, AB; Rich, JN
Cancer Research null
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