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MAB4419

Sigma-Aldrich

Anti-OCT-4 [POU5F1] Antibody, clone 7F9.2

clone 7F9.2, from mouse

别名:

Octamer-binding transcription factor 3, POU class 5 homeobox 1, POU domain class 5, transcription factor 1, POU-type homeodomain-containing DNA-binding protein, octamer-binding transcription factor-3

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100 ML
¥211.05
500 ML
¥665.04
6 X 500 ML
¥2,434.10
24 X 500 ML
¥11,932.77

¥211.05

国内现货，预计发货时间2025年4月14日详情

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变更视图
100 ML
¥211.05
500 ML
¥665.04
6 X 500 ML
¥2,434.10
24 X 500 ML
¥11,932.77

About This Item

UNSPSC代码:
12352203
eCl@ss:
32160702
NACRES:
NA.41

¥211.05

国内现货，预计发货时间2025年4月14日详情

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获取大包装报价

生物来源

mouse

质量水平

100

抗体形式

purified immunoglobulin

抗体产品类型

primary antibodies

克隆

7F9.2, monoclonal

种属反应性

human, mouse

技术

flow cytometry: suitable
immunocytochemistry: suitable
western blot: suitable

输入

sample type: human embryonic stem cell(s)
sample type induced pluripotent stem cell(s)
sample type: mouse embryonic stem cell(s)

同位素/亚型

IgG1κ

NCBI登记号

NP_976034

相关类别

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此商品
PHR1419V900220100684
硝酸盐试剂 B suitable for microbiology

39441

硝酸盐试剂 B

对氨基苯磺酸 Pharmaceutical Secondary Standard; Certified Reference Material

PHR1419

对氨基苯磺酸

磺胺酸 Vetec™, reagent grade, 99%

V900220

磺胺酸

Sulfanilic acid for elemental analysis

100684

Sulfanilic acid

shelf life

limited shelf life, expiry date on the label

shelf life

-

shelf life

-

shelf life

-

composition

acetic acid (30%), 1000 mL

composition

-

composition

-

composition

-

technique(s)

microbe id | metabolite detection: suitable

technique(s)

HPLC: suitable, gas chromatography (GC): suitable

technique(s)

-

technique(s)

-

application(s)

agriculture
clinical testing
environmental
food and beverages
pharmaceutical

application(s)

pharmaceutical (small molecule)

application(s)

-

application(s)

-

suitability

Enterobacter spp.

suitability

-

suitability

-

suitability

-

一般描述

Octamer-4 (Oct-4), a member of the POU family of transcription factors, has been demonstrated to be vital for the formation of self-renewing pluripotent stem cells. During embryogenesis, expression of Oct-4 is limited to pluripotent cells of the inner cell mass (ICM) that contribute to the formation of all fetal cell types. This relationship between Oct-4 and pluripotency has seen this transcription factor emerge as a marker of pluripotent stem cells. Undifferentiated human and murine pluripotent Embryonic Stem (ES) and Embryonic Carcinoma (EC) cells express Oct-4. Additionally, murine Embryonic Germ (EG) cells are also known to express Oct-4. Following stem cell differentiation, the level of Oct-4 expression decreases. Oct-4 expression by human EG cells is not known.

特异性

Reactivity to other species has not been determined.
This antibody reacts with the intracellular protein, Oct-4 and detects the Oct-4A isoform.

免疫原

Recombinant human Oct-4 protein, a.a. 1-145.

应用

Research Category
Stem Cell Research
Research Sub Category
Pluripotent & Early Differentiation
This Anti-OCT-4 [POU5F1] Antibody, clone 7F9.2 is validated for use in IC, FC, WB for the detection of OCT-4 [POU5F1].
Western Blotting: Recommended working dilution is 1 - 2 µg/mL.

Flow Cytometry: Recommended working dilution is 2 µg/mL.

Immunocytochemistry: Recommended working dilution is 1:100 – 1: 200.

目标描述

~39 kDa

联系

Replaces: MAB4305

外形

Protein G sepharose column
Format: Purified
Purified mouse monoclonal IgG1κ in a buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl, and 0.05% NaN3.

储存及稳定性

Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

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    Molecular signatures to define spermatogenic cells in common marmoset (Callithrix jacchus).
    Lin, ZY; Imamura, M; Sano, C; Nakajima, R; Suzuki, T; Yamadera, R; Takehara, Y; Okano et al.
    Reproduction (Cambridge, England) null
    Teresa Starzyńska et al.
    Journal of cellular and molecular medicine, 17(6), 792-799 (2013-05-16)
    Various experimental studies indicate potential involvement of bone marrow (BM)-derived stem cells (SCs) in malignancy development and progression. In this study, we comprehensively analysed systemic trafficking of various populations of BM-derived SCs (BMSCs), i.e., mesenchymal, haematopoietic, endothelial stem/progenitor cells (MSCs
    Foster Birnbaum et al.
    NPJ Regenerative medicine, 7(1), 19-19 (2022-03-20)
    Duchenne muscular dystrophy (DMD) is a progressive genetic myopathy that leads to heart failure from dilated cardiomyopathy by early adulthood. Recent evidence suggests that tamoxifen, a selective estrogen receptor modulator widely used to treat breast cancer, ameliorates DMD cardiomyopathy. However
    Sara Pijuan-Galitó et al.
    The Journal of biological chemistry, 289(48), 33492-33502 (2014-10-11)
    We have previously demonstrated that the Src family kinase Yes, the Yes-associated protein (YAP) and TEA domain TEAD2 transcription factor pathway are activated by leukemia inhibitory factor (LIF) and contribute to mouse embryonic stem (mES) cell maintenance of pluripotency and
    Gisele C M Dias et al.
    Molecular reproduction and development (2020-06-20)
    The objective of this study was to establish a protocol for the characterization, isolation, and culture of type A spermatogonia using specific molecular markers for these cells in fish. To this end, adult Prochilodus lineatus testes were collected and digested
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