AB5603
Anti-SOX2 Antibody
CHEMICON®, rabbit polyclonal
别名:
Transcription factor SOX-2, Sex determining region Y-box 2, SRY-related HMG-box gene 2, Transcription factor SOX2
产品名称
抗Sox2抗体, Chemicon®, from rabbit
biological source
rabbit
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human, mouse
species reactivity (predicted by homology)
rhesus macaque (based on 100% sequence homology), rat (based on 100% sequence homology), bovine (based on 100% sequence homology)
packaging
antibody small pack of 25 μg
manufacturer/tradename
Chemicon®
technique(s)
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
ambient
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
human ... SOX2(6657)
Analysis Note
免疫印迹：小鼠或人类胚胎干细胞裂解液、小鼠胚胎生殖细胞裂解液
免疫细胞化学：人胚胎干细胞（H9细胞系）
蛋白质印迹分析：1 µg/mL的该抗体在10 µg小鼠胚胎干细胞裂解液中检测到SOX2。
Application
免疫细胞化学分析：一个代表性批次通过荧光免疫细胞化学，在4％低聚甲醛固定的，0.1％Triton X-100通透的人类胚胎干细胞（hESCs）中检测到核Sox2的免疫反应性（Kingham，E.，et al.（2013）。Small.9（12）：2140-2151）。
免疫细胞化学分析：一个代表性批次通过荧光免疫细胞化学染色，在从人肾小管肾上皮细胞产生的经4％低聚甲醛固定的iPSC中检测到Sox2的免疫反应性（Montserrat，N.，et al.（2012）。J. Biol. Chem. 287(29):24131-24138).
免疫细胞化学分析: 一个代表性批次通过荧光免疫细胞化学染色，在从帕金森′病患者皮肤细胞产生的经4％低聚甲醛固定、0.5％Triton X-100透化的iPSC中检测到Sox2的免疫反应性(Sánchez-Danés, A., et al. (2012)。EMBO Mol.Med. 4(5):380-395).
免疫细胞化学分析: 一个代表性批次通过荧光免疫细胞化学染色，在从小鼠耳细胞和MEF中产生的经4％低聚甲醛固定、0.2％Triton X-100透化的iPSC中检测到Sox2的免疫反应性（Burns，JC，et al.（2012）。PLoS One.7(10):e48704; Hadadeh, O., et al. (2012).PLoS One.7(11):e49065).
蛋白质印迹分析: 一个代表性批次在小鼠胚胎干细胞（mESC）核提取物中检测到Sox2(Banaszynski, L.A., et al. (2013)。Cell.155（1）：107-120）。
免疫荧光分析：一个代表性批次通过荧光免疫组织化学，在4％低聚甲醛固定、0.3％Triton X-100透化的小鼠脑纤维瘤切片中检测到Sox2阳性细胞（Matsuda，S.，et al.（2012）。J. Neurosci. 32(36):12543-12557).
免疫组织化学分析: 一个代表性批次在4％低聚甲醛固定的整装小鼠胚胎切片中检测到Sox2的免疫反应性（van Rooijen，C.，et al.Development.139(14):2576-2583）。
Biochem/physiol Actions
General description
Immunogen
Legal Information
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
商品
Human iPSC neural differentiation media and protocols used to generate neural stem cells, neurons and glial cell types.
ReNcell neural progenitors are immortalized human neural stem cell lines that can differentiate into neurons, astrocytes sand oligodendrocytes.
实验方案
Step-by-step culture protocols for neural stem cell culture including NSC isolation, expansion, differentiation and characterization.
相关内容
抗体的质量决定了整个免疫检测的成败， 是在选择抗体时首选的考虑因素。基于Sigma®、Chemicon®、Upstate®、Mil l ipore®、 Calbiochem®和Novagen®等子品牌的强强联合，默克在创新的 免疫原设计、免疫、挑选、筛选和验证等方面有着数十年的经验， 创造出许多被广泛使用的抗体，如4G10，Neun，组蛋白修饰等抗 体都是相关领域的“金标”抗体。 我们的技术和科研背景使默克成为高质量抗体的主要设计者和生产 者，而不仅仅是经销商。毋庸置疑，我们的每个小瓶中的抗体都包 含着大量的科学研究成果。
As the focus of stem cell research undergoes a transition from animal to human models, researchers are in critical need of validated products to support the isolation, maintenance, differentiation, and characterization of human stem cells. While many reagents designed for rodent systems can be applied to human stem cell studies, they are not truly optimized for robust human stem cell culture or analysis. This is why human stem cell researchers have always trusted EMD Millipore, the first provider of commercially available human embryonic stem cells and human neural stem cell lines, to accelerate their research. All of our human stem cell systems are extensively tested in defined media culture, and differentiated progeny are comprehensively characterized with highly validated antibodies and detection reagents.
Millipore’s new STEMCCA lentivirus reprogramming kits make it easier than ever to generate induced pluripotent stem (iPS) cells. Unlike traditional iPS generation which requires simultaneous co-infection by four separate expression vectors, the STEMCCA kits use a single polycistronic lentiviral vector to improve efficiency and reduce the number of viral integrations. The STEMCCA vector is comprised of the transcription factors Oct-4, Klf4, SOX-2, and c-Myc (OKSM), separated by the self-cleaving 2A peptide and IRES sequences 1,2. It is also available with flanking LoxP sites incorporated for Cre-mediated excision of the exogenous reprogramming transgenes. STEMCCA Vector Advantages: (1) Efficient: uses a single vector with four transcription factors rather than co-transducing four separate expression vectors (2) Minimizes viral integrations: single vector reduces the risks of insertional mutagenesis and viral reactivation and (3) Excisable: Cre/LoxP-regulated version enables removal of reprogramming transgenes.
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