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产品名称
Origami B(DE3) 感受态细胞 - Novagen, Origami B host strains carry the same mutations as the original Origami strain, except that they are derived from a lacZY mutant of BL21 to enable precise control of expression levels using IPTG.
生物来源
Escherichia coli
质量水平
制造商/商品名称
Novagen®
储存条件
OK to freeze
生长模式
adherent or suspension
形态学
rod shaped
技术
microbiological culture: suitable
细胞转化
transformation efficiency: >2×106 cfu/μg
运输
dry ice
储存温度
−70°C
相关类别
一般描述
基因型：F-ompT hsdSB(rB- mB-) gal dcm lacY1 ahpC（DE3) gor522::Tn10 trxB（KanR，TetR)
该产品包含转基因生物(GMO)。在欧盟范围内，转基因物种分别受欧洲议会和理事会的2001/18 /EC和2009/41/EC指令及其成员国中国家所实施规范的约束。该法规规定我们有义务索取您以及处理转基因生物的机构的相关信息。请点击here获得最终用户声明（EUD）表格。
该产品包含转基因生物(GMO)。在欧盟范围内，转基因物种分别受欧洲议会和理事会的2001/18 /EC和2009/41/EC指令及其成员国中国家所实施规范的约束。该法规规定我们有义务索取您以及处理转基因生物的机构的相关信息。请点击here获得最终用户声明（EUD）表格。
Origami B宿主菌株在trxB和gor中携带与原始Origami菌株相同的突变，只是它们来自BL21的lacZY突变体，可以通过调节IPTG的浓度来精确控制表达水平。因此Origami B菌株在一个菌株背景中结合了BL21、Tuner和Origami菌株的理想特征。trxB和gor的突变分别在卡那霉素和四环素上是可选择的；因此，这些菌株不能与只能用卡那霉素或四环素选择的质粒一起使用。这些菌株还包括BL21的lon和ompT缺陷，可增加蛋白质的稳定性。
DE3表明宿主是λDE3的溶原菌，因此在lacUV5启动子的控制下携带T7 RNA聚合酶基因的染色体拷贝。这样的菌株适用于通过用IPTG诱导从克隆在pET载体中的靶基因生产蛋白质。
DE3表明宿主是λDE3的溶原菌，因此在lacUV5启动子的控制下携带T7 RNA聚合酶基因的染色体拷贝。这样的菌株适用于通过用IPTG诱导从克隆在pET载体中的靶基因生产蛋白质。
Origami B宿主菌株携带与原始Origami菌株相同的突变，不同之处在于它们源自BL21的lacZY突变体，可以使用IPTG精确控制表达水平。
组分
0.4 ml1 ml组分
•2 × 0.2 ml5 × 0.2 mlOrigami B(DE3)感受态细胞
•2 × 2 ml4 × 2 mlSOC培养基
•2 ng2 ng供试质粒
•2 × 0.2 ml5 × 0.2 mlOrigami B(DE3)感受态细胞
•2 × 2 ml4 × 2 mlSOC培养基
•2 ng2 ng供试质粒
警告
毒性：多个毒性值，请参阅MSDS（O）
法律信息
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
储存分类代码
10 - Combustible liquids
WGK
WGK 2
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