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70836-M

Sigma-Aldrich

Origami B Competent Cells - Novagen

Origami B host strains carry the same mutations as the original Origami strain, except that they are derived from a lacZY mutant of BL21 to enable precise control of expression levels using IPTG.

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UNSPSC代码:
41106202
NACRES:
NA.81

生物来源

Escherichia coli

质量水平

制造商/商品名称

Novagen®

储存条件

OK to freeze

生长模式

adherent or suspension

形态学

rod shaped

技术

microbiological culture: suitable

细胞转化

transformation efficiency: >2×106 cfu/μg

运输

dry ice

储存温度

−70°C

一般描述

Genotype: F-ompT hsdSB(rB- mB-) gal dcm lacY1 ahpC gor522::Tn10 trxB (KanR, TetR)





This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges us to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
Origami B host strains carry the same trxB/gor mutations as the original Origami strains, except that they are derived from a lacZY mutant of BL21. Thus the Origami B strains combine the desirable characteristics of BL21, Tuner and Origami hosts in one strain background. The trxB and gor mutations are selectable on kanamycin and tetracycline, respectively; therefore, these strains are recommended for use only with pET plasmids carrying the ampicillin resistance marker bla.
Origami B host strains carry the same mutations as the original Origami strain, except that they are derived from a lacZY mutant of BL21 to enable precise control of expression levels using IPTG.

组分

0.4 ml1 mlComponent

•2 × 0.2 ml5 × 0.2 mlOrigami B Competent Cells

•2 × 2 ml4 × 2 mlSOC Medium

•10 µl10 µlTest Plasmid

警告

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

法律信息

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

储存分类代码

10 - Combustible liquids

WGK

WGK 2


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