产品名称
Rosetta(DE3)感受态细胞 - Novagen, Rosetta host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli.
biological source
Escherichia coli
manufacturer/tradename
Novagen®
storage condition
OK to freeze
growth mode
adherent or suspension
morphology
rod shaped
technique(s)
microbiological culture: suitable
shipped in
wet ice
storage temp.
−70°C
Quality Level
Disclaimer
毒性：多个毒性值，请参阅MSDS（O）
General description
Rosetta宿主菌株是BL21衍生物，旨在增强真核蛋白的表达，该蛋白含有在大肠杆菌中很少使用的密码子。这些菌株在兼容的氯霉素抗性质粒上为AGG、AGA、AUA、CUA、CCC、GGA密码子提供tRNA。因此，Rosetta菌株提供了“通用”翻译，否则该翻译受大肠杆菌密码子使用的限制。tRNA基因由其天然启动子驱动。在Rosetta（DE3）pLysS中，稀有tRNA基因存在于携带T7溶菌酶基因的相同质粒上。
DE3表明宿主是λDE3的溶原菌，因此在lacUV5启动子的控制下携带T7 RNA聚合酶基因的染色体拷贝。这样的菌株适用于通过用IPTG诱导从克隆在pET载体中的靶基因生产蛋白质。
DE3表明宿主是λDE3的溶原菌，因此在lacUV5启动子的控制下携带T7 RNA聚合酶基因的染色体拷贝。这样的菌株适用于通过用IPTG诱导从克隆在pET载体中的靶基因生产蛋白质。
基因型：F-ompT hsdSB(rB- mB-) gal dcm (DE3) pRARE (CamR)
该产品包含转基因生物(GMO)。在欧盟范围内，转基因物种分别受欧洲议会和理事会的2001/18 /EC和2009/41/EC指令及其成员国中国家所实施规范的约束。该法规规定我们有义务索取您以及处理转基因生物的机构的相关信息。请点击这里获得最终用户声明（EUD）表格。
该产品包含转基因生物(GMO)。在欧盟范围内，转基因物种分别受欧洲议会和理事会的2001/18 /EC和2009/41/EC指令及其成员国中国家所实施规范的约束。该法规规定我们有义务索取您以及处理转基因生物的机构的相关信息。请点击这里获得最终用户声明（EUD）表格。
Rosetta宿主菌株是BL21衍生物，旨在增强真核蛋白的表达，该蛋白含有在大肠杆菌中很少使用的密码子。
Other Notes
0.4 ml1 ml组分
•2 × 0.2 ml5 × 0.2 mlRosetta(DE3)感受态细胞
•2 × 2 ml4 × 2 mlSOC培养基
•2 ng2 ng供试质粒
•2 × 0.2 ml5 × 0.2 mlRosetta(DE3)感受态细胞
•2 × 2 ml4 × 2 mlSOC培养基
•2 ng2 ng供试质粒
Legal Information
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
存储类别
10 - Combustible liquids
wgk
WGK 2
Xiangyu Liu et al.
Cell, 177(5), 1243-1251 (2019-05-14)
The crystal structure of the β2-adrenergic receptor (β2AR) bound to the G protein adenylyl cyclase stimulatory G protein (Gs) captured the complex in a nucleotide-free state (β2AR-Gsempty). Unfortunately, the β2AR-Gsempty complex does not provide a clear explanation for G protein
Caitlin I Stoddard et al.
Molecular cell, 73(1), 73-83 (2018-11-13)
DNA methylation and H3K9me are hallmarks of heterochromatin in plants and mammals, and are successfully maintained across generations. The biochemical and structural basis for this maintenance is poorly understood. The maintenance DNA methyltransferase from Zea mays, ZMET2, recognizes dimethylation of
Koning Shen et al.
Molecular cell, 74(4), 729-741 (2019-04-16)
The nascent polypeptide-associated complex (NAC) is a conserved ribosome-associated protein biogenesis factor. Whether NAC exerts chaperone activity and whether this function is restricted to de novo protein synthesis is unknown. Here, we demonstrate that NAC directly exerts chaperone activity toward
Emily Anne Scarborough et al.
eLife, 8 (2019-05-03)
Regulation of the outer kinetochore complex Ndc80 is essential to ensure correct kinetochore-microtubule attachments during mitosis. Here, we present a novel mechanism of regulation that is intrinsic to its structure; tight bending of the Ndc80 complex inhibits its microtubule binding.
Naomichi Takemata et al.
Cell, 179(1), 165-179 (2019-09-21)
The three-dimensional organization of chromosomes can have a profound impact on their replication and expression. The chromosomes of higher eukaryotes possess discrete compartments that are characterized by differing transcriptional activities. Contrastingly, most bacterial chromosomes have simpler organization with local domains
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