product name
过表达™ C41 (DE3) 化学感受态细胞, for the highest protein expression
生物来源
Escherichia coli
等级
for molecular biology
生长模式
adherent or suspension
形态学
rod shaped
技术
microbiological culture: suitable
细胞转化
competent cell type: chemically competent
transformation efficiency: ≥1 x 106 cfu/μg
运输
dry ice
储存温度
−70°C
相关类别
一般描述
OverExpress™ Electrocompetent and Chemically Competent Cells是大肠杆菌菌株，可有效表达所有种类生物体（包括真细菌、酵母、植物、病毒和哺乳动物）中的有毒蛋白质。这些新菌株在表达有毒蛋白质方面的有效性已在350多篇论文中得到验证。OverExpress™菌株含有从表型上选择的基因突变，以赋予对有毒蛋白质的耐受性。菌株C41（DE3）来自BL21（DE3）。该菌株至少有一个突变，其防止了与许多重组有毒蛋白表达相关的细胞死亡。菌株C43（DE3）来源于C41（DE3），通过选择对不同毒性蛋白的抗性而得到，它可以表达C41（DE3）的一系列不同的有毒蛋白。OverExpress™ C41(DE3)pLysS和 C43(DE3)pLysS还带有氯霉素抗性质粒，该质粒编码T7 RNA聚合酶的天然抑制剂T7溶菌酶。含有pLysS的细胞会产生少量的T7溶菌酶。这些菌株可用于在诱导之前抑制T7 RNA聚合酶的基础表达，从而使编码特别有毒的蛋白质的重组体保持稳定。
基因型
F – ompT hsdSB (rB- mB-) gal dcm (DE3)
基因型
F – ompT hsdSB (rB- mB-) gal dcm (DE3)
应用
OverExpress™ C43(DE3)化学感受态细胞已用于转化包含SAM和TIR域的蛋白1(sterile alpha and TIR motif–containing protein 1,SARM1)的toll/白介素受体(TIR)结构域构建体。
特点和优势
- 用这些 BL21 (DE3) 衍生物克隆到任何 T7 载体的表达基因
- 有效表示毒性和膜蛋白类
- 被超过 350 篇研究文章引用
组分
- OverExpress C43(DE3) 化学感受态细胞
- pUC 19 转化对照 DNA
- 表达回收培养基
法律信息
OverExpress is a trademark of Imaxio, S.A.
相关产品
产品编号
说明
价格
WGK
WGK 3
法规信息
高风险级别生物产品--病毒，疫苗等
Solène N Lefebvre et al.
eLife, 10 (2021-10-01)
Pentameric ligand-gated ion channels (pLGICs) mediate chemical signaling through a succession of allosteric transitions that are yet not completely understood as intermediate states remain poorly characterized by structural approaches. In a previous study on the prototypic bacterial proton-gated channel GLIC
Jia-Qi Lu et al.
International journal of molecular sciences, 22(21) (2021-11-14)
Ribosome-inactivating proteins (RIPs) hydrolyze the N-glycosidic bond and depurinate a specific adenine residue (A-4324 in rat 28S ribosomal RNA, rRNA) in the conserved α-sarcin/ricin loop (α-SRL) of rRNA. In this study, we have purified and characterized lyophyllin, an unconventional RIP
A Rangunwala et al.
Epidemiology and infection, 142(10), 2147-2154 (2013-12-18)
Crimean-Congo haemorrhagic fever virus (CCHFV) has the propensity to cause nosocomial infections with a high fatality rate. Handling the virus requires biosafety level-4 facilities, limiting accessibility for many laboratories. Advances in molecular techniques have allowed preparation of safe recombinant antigens
Heather S Loring et al.
Bioorganic & medicinal chemistry, 28(18), 115644-115644 (2020-08-24)
Sterile Alpha and Toll Interleukin Receptor Motif-containing protein 1 (SARM1) is a key therapeutic target for diseases that exhibit Wallerian-like degeneration; Wallerian degeneration is characterized by degeneration of the axon distal to the site of injury. These diseases include traumatic
Arthur Laganowsky et al.
Nature, 510(7503), 172-175 (2014-06-06)
Previous studies have established that the folding, structure and function of membrane proteins are influenced by their lipid environments and that lipids can bind to specific sites, for example, in potassium channels. Fundamental questions remain however regarding the extent of
实验方案
Protocol for OverExpress™ Chemically Competent Cells. Product Numbers: CMC0017, CMC0018, CMC0019, CMC0020, CMC0023, CMC0024
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