Rosetta-gami B(DE3) Competent Cells - Novagen

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

Genotype: F^–ompT hsdS_B (r_B^– m_B^–) gal dcm lacY1 ahpC (DE3) gor522::Tn10 trxB pRARE (Cam^R, Kan^R, Tet^R)

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Rosetta-gami B strains combine the key features of BL21 (and its Tuner derivative), Origami, and Rosetta to enhance both the expression of eukaryotic proteins and the formation of target protein disulfide bonds in the bacterial cytoplasm. These strains are compatible with ampicillin- or spectinomycin-resistant vectors.

DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.

Rosetta-gami B strains combine the key features of BL21, Origami, and Rosetta to enhance both the expression of eukaryotic proteins and the formation of target protein disulfide bonds.

0.4 ml1 mlComponent

•2 × 0.2 ml5 × 0.2 mlRosetta-gami B(DE3) Competent Cells

•2 × 2 ml4 × 2 mlSOC Medium

•10 µl10 µlTest Plasmid

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