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安全信息

T9424

Sigma-Aldrich

TRI试剂®

For processing tissues, cells cultured in monolayer or cell pellets

别名:

TRI试剂® RNA 分离试剂

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About This Item

MDL编号:
MFCD00213058
UNSPSC代码:
12352200
NACRES:
NA.52

质量水平

200

用途

 mL sufficient for 107 cells
 mL sufficient for 100 mg tissue (or)

储存温度

2-8°C

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相关类别

一般描述

TRI reagent®是一种快速方便的即用型试剂，可实现有效的总RNA提取或同时分离RNA、DNA和蛋白质。TRI reagent®是溶于单相溶液的硫氰酸胍和苯酚混合物。
组织样品在TRI reagent®中均质或裂解，即可溶解RNA、DNA和蛋白质。加入氯仿或1-溴-3-氯丙烷，然后离心，混合物将分离为三层：含RNA的水相、含DNA的中间相和含蛋白质的有机相。随后即可通过分离不同相层，得到相应组分。

应用

TRI Reagent已用于：
  • 从各种组织样品和细胞中分离总RNA
  • 裂解单核细胞
  • 从感染SARS-CoV的Vero细胞中分离细胞质RNA
  • 从感染甲型流感(H5N1)病毒的MDM（单核细胞衍生的巨噬细胞）中分离总RNA
  • 制备SARS-CoV培养物的病毒裂解液，以便为SARS-CoV疫苗开发创建编码S糖蛋白的克隆

使用TRI reagent®分离的RNA、DNA和蛋白质可继续用于各种下游应用，如克隆、PCR、RT-PCR、Northern印迹、mRNA分离、体外翻译、RNase保护测定、限制酶消化、Southern印迹、SDS-PAGE和蛋白质印迹。
TRI试剂是对由Chomczynski开发的一步法总RNA分离试剂进行改良的一种版本。基于该试剂的RNA分离方法已被广泛用于和验证用于RNA应用。它是对来自人、动物、植物、酵母、细菌和病毒来源样本进行总RNA或RNA、DNA和蛋白同时分离的一种理想的快速、经济和高效的方法。

特点和优势

易于使用：通过一种试剂可同时分离RNA、DNA和蛋白质，不会残留任何污染物。
多用途：TRI Reagent®同时适于大量或少量组织或细胞，同时支持多种类型的样品，包括人、植物、酵母、细菌和病毒来源样品。
高效：相对于传统硫氰酸胍/氯化铯方法，TRI reagent®产量更高。从新鲜组织或细胞开始的完整RNA提取流程可在一小时内完成。
  • 可轻松扩展的RNA分离
  • 可适用于多种来源：人、植物、酵母、细菌或病毒
  • 相对于传统的硫氰酸胍/氯化铯具有更高的产量

其他说明

2024年CiteAb奖——成功的帕金森研究供应商(2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research)

法律信息

TRI Reagent is a registered trademark of Molecular Research Center, Inc.

警示用语：

Danger

危险分类

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2

靶器官

Nervous system,Kidney,Liver,Skin

补充剂危害

EUH032

WGK

WGK 2

闪点(°F)

174.2 °F

闪点(°C)

79 °C

法规信息

危险化学品

分析证书(COA)

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  1. My cells are plated on polystyrene culture plates. How do I use Product T9424, TRI Reagent in this application?

    We recommend harvesting the cells in PBS with a rubber policeman/cell scraper, transfer to an eppendorf tube, and proceed with the RNA isolation by removing the PBS from the cell pellet and re-suspending the pellet in the Tri Reagent.

  2. If I add a volume of Product T9424, Tri Reagent™, different from 1 ml, how much chloroform and isopropanol should I add?

    What is important is to keep the ratio the same. For example, we recommend adding 200 μL of chloroform and 500 μL of isopropanol when using 1 ml of Tri Reagent. Therefore, 100 μL of chloroform and 250 μL of isopropanol would be added if using 500 μL of Tri Reagent. Similarly, 300 μL of chloroform and 750 μL of isopropanol would be added to if using 1.5 ml of Tri Reagent.

  3. What is the expiration date for Product T9424, Tri Reagent™?  

    The expiration date for T9424 has not been established. However, based on our storage guidelines, it should be usable for 1 year from the date of receipt.

  4. Can I use T9424 Tri Reagent™ for blood samples?

    No, we recommend using T3809 Tri Reagent BD for work in blood samples.

  5. Which chloroform and isopropanol do you recommend to be used with T9424, Tri Reagent™?

    For chloroform and isopropanol we recommend C2432 and I9616, respectively.

  6. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  7. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  8. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  9. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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Kim KS
Journal of Neurochemistry, 107(1), 105-115 (2008)
Defining the importance of phosphatidylserine synthase 2 in mice.
Bergo MO
The Journal of Biological Chemistry, 277 (2002)
Soo Hee Kim et al.
Oncotarget, 8(19), 31305-31317 (2017-04-17)
Recently, various long non-coding RNAs (lncRNAs) have been reported to have significant therapeutic or prognostic value. However, the expression of lncRNAs has not been investigated in T and NK cell lymphoma. Thus, we evaluated the biological and prognostic role of
Lack of SIRT1 (Mammalian Sirtuin 1) activity leads to liver steatosis in the SIRT1+/- mice: a role of lipid mobilization and inflammation.
Xu F
Endocrinology (2010)
E M Menkhorst et al.
Scientific reports, 7(1), 8690-8690 (2017-08-20)
During the establishment of pregnancy, extravillous trophoblast (EVT) must invade into the uterine decidua to facilitate decidual artery remodelling to create the placental blood supply. The local decidual environment is thought to regulate trophoblast invasion, however these interactions are poorly
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