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安全信息

R0901

Sigma-Aldrich

RNAlater®

Stabilize and protect RNA with immediate RNase inactivation

别名:

RNA preservation solution, RNA protection solution, RNA protector, RNA stabilizer, RNA storage solution, RNA transportation solution

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About This Item

MDL编号:
MFCD03453003
UNSPSC代码:
12352200
NACRES:
NA.52

质量水平

100

SMILES字符串

N([C@@H]([C@H](O)C)C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](CCCNC(=N)N)C(=O)O)C(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](N)CCCNC(=N)N)CC(=O)N)C)CC(C)C)C

InChI

1S/C37H67N15O13/c1-16(2)14-23(50-28(57)17(3)46-33(62)24(15-25(39)54)51-30(59)20(38)8-6-12-44-36(40)41)32(61)47-18(4)29(58)52-27(19(5)53)34(63)48-21(10-11-26(55)56)31(60)49-22(35(64)65)9-7-13-45-37(42)43/h16-24,27,53H,6-15,38H2,1-5H3,(H2,39,54)(H,46,62)(H,

InChI key

DUCQRBNNHJNRMP-KDDVYCELSA-N

相关类别

一般描述

RNAlater与绝大多数的RNA分离方法相兼容，包括TRI试剂和GenElute总RNA分离和哺乳动物mRN分离试剂盒。

RNAlater是一种水相的无毒性组织储存试剂，可在未冷冻的样本中快速穿透组织以在原位对细胞RNA进行稳定和保护。 组织块在收集后立即浸没在RNAlater中进行储存，而不会损害RNA的质量或数量。RNAlater免去了对组织样本立即进行处理或冷冻在液氮中用于后续处理的需要。 RNAlater在37 °C、25 °C和4 °C保存组织中的RNA分别长达1天、一周和一个月。组织还可在-20 °C保存更长时间。

RNAlater已利用来自脊椎动物包括大脑、心脏、肾脏、脾脏、肝脏、睾丸、骨骼肌、脂肪、肺和胸腺的多种组织进行广泛的测试。RNAlater还可有效用于大肠杆菌、果蝇、组织培养细胞、白细胞和某些植物。
RNAlater是一种水相的无毒性组织储存试剂，可在未冷冻的样本中快速穿透组织以在原位对细胞RNA进行稳定和保护。 组织块在收集后立即浸没在RNAlater中进行储存，而不会损害RNA的质量或数量。RNAlater免去了对组织样本立即进行处理或冷冻在液氮中用于后续处理的需要。 RNAlater在37 °C、25 °C和4 °C保存组织中的RNA分别长达1天、一周和一个月。组织还可在-20 °C保存更长时间。

RNAlater可用于各种下游应用包括mRNA及总RNA的分离、组织学和免疫组化，并与Sigma的分离试剂盒相兼容。

应用

RNAlater®已用于合成反义RNA探针。它可用于各种下游应用包括mRNA及总RNA分离、组织学和免疫组合，并与Sigma的GenElute™分离试剂盒相兼容。

特点和优势

  • 不会对后续mRNA或总RNA的分离造成RNA质量的影响
  • 快速穿透组织已稳定并保护细胞RNA
  • 可实现下游组织处理的水相无毒性溶液
  • 在室温对样本进行稳定

其他说明

RNAlater®是一种水相的无毒性组织和细胞储存试剂，可对完整的、未冷冻的组织和细胞样本中的细胞RNA进行保护。 RNAlater免去了对样本立即进行处理或冷冻在液氮中用于后续处理的需要。

原理

RNAlater易于使用。仅需简单地将待储存的组织样品切割成在至少一个维度上小于0.5 cm，并浸没在5倍体积的RNAlater中。小的器官，如大鼠肾脏、肝脏或脾脏可被整体储存在RNAlater中。当准备好需要分离RNA时，可从RNAlater 中取出组织并按照像刚收集到的组织一样进行处理。对于细胞储存，可在加入5-10倍体积的RNAlater之前使用少量的PBS用于重悬沉淀的细胞。在制备RNA之前，将细胞进行沉淀并弃上清。

法律信息

获得了Ambion, Inc., The RNA Company授权进行分销。
GenElute is a trademark of Sigma-Aldrich Co. LLC
RNAlater is a registered trademark of Ambion, Inc.

通常也和此产品一起购买

产品编号
说明
价格

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

监管及禁止进口产品

从最新的版本中选择一种：

分析证书(COA)

Lot/Batch Number
MKCW5003
MKCT9074
MKCT6402
MKCQ5236
MKCR6534

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如果您需要特殊版本，可通过批号或批次号查找具体证书。

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What is the shelf life of R0901, RNAlater®?

    R0901 does not have a specified retest or expiration date.  We guarantee the product for 1 year from the date of receipt, according to our Product Dating and Shipping document.

  4. How do you recommend I store my samples in Product R0901, RNAlater®?

    RNAlater preserves RNA in tissues for up to 1 day at 37 °C, 1 week at 25 °C, and 1 month or more at 4 °C. Tissues can also be stored at -20 °C or at -80 °C long-term.

  5. When using Product R0901, RNAlater® I observed crystal formation in the sample after freezing.  Is there any way to avoid this?

    Yes, incubate samples at 4 °Covernight, then remove them from RNAlater before storage at -80 °C to prevent the formation of salt crystals.

  6. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  7. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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The Wnt pathway regulator DKK1 is preferentially expressed in hormone-resistant breast tumours and in some common cancer types.
Forget MA
British Journal of Cancer, 96(4), 646-653 (2007)
Tina Jorsal et al.
Diabetologia, 61(2), 284-294 (2017-09-29)
Enteroendocrine K and L cells are pivotal in regulating appetite and glucose homeostasis. Knowledge of their distribution in humans is sparse and it is unknown whether alterations occur in type 2 diabetes. We aimed to evaluate the distribution of enteroendocrine
Jaret P Bilewitch et al.
BMC evolutionary biology, 11, 228-228 (2011-08-02)
The mitochondrial genome of the Octocorallia has several characteristics atypical for metazoans, including a novel gene suggested to function in DNA repair. This mtMutS gene is favored for octocoral molecular systematics, due to its high information content. Several hypotheses concerning
Vitellogenin gene expression in the intertidal blenny Lipophrys pholis: a new sentinel species for estrogenic chemical pollution monitoring in the European Atlantic coast?
Ferreira F
Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP, 149(1), 58-64 (2009)
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