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About This Item
线性分子式:
Cl(CH2)3Br
CAS号:
分子量:
157.44
Beilstein:
605278
EC 号:
MDL编号:
UNSPSC代码:
41105515
PubChem化学物质编号:
NACRES:
NA.52
等级:
for molecular biology
方案:
≥98.5% (GC)
沸点:
144-145 °C (lit.)
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一般描述
BCP毒性较小，可用于代替氯仿。在TRI试剂单步提取方法中使用BCP，可降低RNA被DNA污染的可能性。它对分离所得RNA的质量或数量无不良影响。1-溴-3-氯丙烷是用于核酸分离方案的相分离试剂。
应用
1-溴-3-氯丙烷已用于分子生物学应用，可从下列物质分离高质量RNA：
- 猫牙齿
- 小鼠大脑和肝脏组织
- 人子宫颈外上皮细胞
- 胎盘样品
- 人间充质基质细胞(hMSC)
特点和优势
- 氯仿的低毒性替代品
- 适用于TRI试剂RNA提取法，可降低DNA污染的可能性
其他说明
BCP毒性较小，可用于代替氯仿。在TRI试剂单步提取方法中使用BCP，可降低RNA被DNA污染的可能性。它对分离所得RNA的质量或数量无不良影响。
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警示用语：
Danger
危险分类
Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Muta. 2 - STOT SE 3
靶器官
Respiratory system
储存分类代码
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
WGK
WGK 2
个人防护装备
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
法规信息
危险化学品
Substitution of chloroform by bromo-chloropropane in the single-step method of RNA isolation.
P Chomczynski et al.
Analytical biochemistry, 225(1), 163-164 (1995-02-10)
Differences in X-chromosome transcriptional activity and cholesterol metabolism between placentae from swine breeds from Asian and Western origins
Bischoff SR, et al.
Testing, 8(1), e55345-e55345 (2013)
Ghalia Boubaker et al.
PLoS neglected tropical diseases, 14(5), e0007640-e0007640 (2020-05-23)
We present a comprehensive analysis of the hepatic miRNA transcriptome at one month post-infection of experimental primary alveolar echinococcosis (AE), a parasitic infection caused upon ingestion of E. multilocularis eggs. Liver tissues were collected from infected and non-infected C57BL/6 mice
ALCAM (CD166) as a gene expression marker for human mesenchymal stromal cell characterisation
Brinkhof, et al.
Gene: X, 5, 100031-100031 (2020)
Estradiol alters the immune-responsiveness of cervical epithelial cells stimulated with ligands of Toll-like receptors 2 and 4
Lashkari BS and Anumba DOC
PLoS ONE, 12(3), e0173646-e0173646 (2017)
实验方案
TRI试剂是一种单步RNA分离试剂，可同时分离DNA、RNA和蛋白质。本实验方案概述了样品制备方法，以及各种样品提取方法和故障排除方案。
TRI Reagent enables simultaneous DNA, RNA, and protein isolation with sample prep guidelines and troubleshooting.
Method for purification, reverse transcription and quantitative PCR for MicroRNAs using Mysticq reagents
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