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安全信息

T3809

Sigma-Aldrich

TRI试剂®

BD, For processing whole blood, plasma, or serum.

别名:

TRI试剂® RNA 分离试剂

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选择尺寸

25 μL
¥2,257.75
100 μL
¥5,169.41
200 μL
¥8,231.20

¥2,257.75

国内现货，预计发货时间2025年4月27日详情

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选择尺寸

变更视图
25 μL
¥2,257.75
100 μL
¥5,169.41
200 μL
¥8,231.20

About This Item

MDL编号:
MFCD00213058
UNSPSC代码:
12352200
NACRES:
NA.52

¥2,257.75

国内现货，预计发货时间2025年4月27日详情

质量水平

200

用途

0.75 mL sufficient for 0.25 mL blood derivatives

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此商品
T5168T6793T6074
Anti-Acetylated Tubulin antibody, Mouse monoclonal clone 6-11B-1, purified from hybridoma cell culture

T7451

Anti-Acetylated Tubulin antibody, Mouse monoclonal

Monoclonal Anti-α-Tubulin antibody produced in mouse clone B-5-1-2, ascites fluid

T5168

Monoclonal Anti-α-Tubulin antibody produced in mouse

Monoclonal Anti-Tubulin, Acetylated antibody produced in mouse clone 6-11B-1, ascites fluid

T6793

Monoclonal Anti-Tubulin, Acetylated antibody produced in mouse

Anti-α-Tubulin antibody, Mouse monoclonal clone B-5-1-2, purified from hybridoma cell culture

T6074

Anti-α-Tubulin antibody, Mouse monoclonal

Gene Information

human ... TUBA4A(7277)
mouse ... Tuba1a(22142)
rat ... Tuba1a(64158)

Gene Information

human ... TUBA4A(7277)
mouse ... Tuba1a(22142)
rat ... Tuba1a(64158)

Gene Information

human ... TUBA4A(7277)
mouse ... Tuba1a(22142)
rat ... Tuba1a(64158)

Gene Information

human ... TUBA4A(7277)
mouse ... Tuba1a(22142)
rat ... Tuba1a(64158)

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

species reactivity

bovine, frog, invertebrates, human, hamster, mouse, protista, pig, monkey, chicken, rat, plant

species reactivity

mouse, chicken, Chlamydomonas, African green monkey, human, rat, bovine, sea urchin, kangaroo rat

species reactivity

mouse, protista, invertebrates, hamster, plant, frog, rat, monkey, pig, bovine, chicken, human

species reactivity

mouse, chicken, Chlamydomonas, African green monkey, human, rat, bovine, sea urchin, kangaroo rat

clone

6-11B-1, monoclonal

clone

B-5-1-2, monoclonal

clone

6-11B-1, monoclonal

clone

B-5-1-2, monoclonal

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody form

ascites fluid

antibody form

ascites fluid

antibody form

purified from hybridoma cell culture

一般描述

TRI reagent®是苯酚和异硫氰酸胍的单相溶液。[1]
TRI试剂BD是一种可用于从血清、血浆或全血中进行RNA、DNA和蛋白同时分离的快速便捷型试剂。便捷的一步液相分离可实现RNA、DNA和蛋白的同时分离。该过程是对由Chomczynski和Sacchi所报道的一步法方法的升级，并可实现对血液衍生物的快速而有效处理。

TRI试剂BD非常适合处理大小体积的样品，且可同时提取多份样品。TRI试剂BD是一种硫氰酸胍和苯酚的单相混合溶液。用它对血液衍生物进行裂解并加入氯仿或1-溴-3-氯丙烷时，混合液会分离为3相：含有RNA的水相、含有DNA的中间相以及含有蛋白的有机相。随后每种组分可在相分离后得到分离。0.75 ml的TRI试剂BD可处理0.25 ml的血液衍生物。

这是分离总RNA最为有效的方法之一，以新鲜细胞起始仅需1小时便能完成。该过程可超高效分离0.1至15 kb的各种RNA分子。得到的RNA可保持完整，并不含或仅含极少量DNA或蛋白。

应用

TRI试剂®已用于分离RNA。[2][3]
TRI试剂是对由Chomczynski开发的一步法总RNA分离试剂进行改良的一种版本。基于该试剂的RNA分离方法已被广泛用于和验证用于RNA应用。它是对来自人、动物、植物、酵母、细菌和病毒来源样本进行总RNA或RNA、DNA和蛋白同时分离的一种理想的快速、经济和高效的方法。
产生的RNA可用于Northern blot、mRNA分离、体外翻译、RNase保护检测、克隆及PCR。

特点和优势

  • 可轻松扩展的RNA分离
  • 可适用于多种来源：人、植物、酵母、细菌或病毒
  • 相对于传统的硫氰酸胍/氯化铯具有更高的产量

法律信息

TRI Reagent is a registered trademark of Molecular Research Center, Inc.

警示用语：

Danger

危险分类

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2

靶器官

Nervous system,Kidney,Liver,Skin

补充剂危害

EUH032

储存分类代码

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 3

闪点(°F)

174.2 °F - closed cup

闪点(°C)

79 °C - closed cup

法规信息

危险化学品

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
MKCQ9520
MKCK7193
MKCH8720
MKCD4813
MKBX1805V

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John-Edwin Thomson et al.
Pancreas, 48(1), 107-112 (2018-11-20)
Interleukin-21 (IL-21) is a cytokine associated with tissue inflammation, autoimmune and infectious diseases. Organ dysfunction and death can occur in patients with acute pancreatitis (AP) in two distinct clinical phases. Initially, a systemic inflammatory response syndrome may be followed by
Heiner Kuhl et al.
Molecular biology and evolution, 38(1), 108-127 (2020-08-12)
Presumably, due to a rapid early diversification, major parts of the higher-level phylogeny of birds are still resolved controversially in different analyses or are considered unresolvable. To address this problem, we produced an avian tree of life, which includes molecular
A novel CLCN5 mutation in a Chinese boy with Dent's disease.
Ji LN
World Journal of Pediatrics : WJP, 10(3), 275-277 (2014)
Circulating ribonucleic acids and metabolic stress parameters may reflect progression of autoimmune or inflammatory conditions in juvenile type 1 diabetes.
Kocic G
TheScientificWorldJournal, 11, 1496-1508 (2011)
Purification of RNA using TRIzol (TRI reagent)
Rio D, et al.
Cold Spring Harbor Protocols, 2010(6), pdb-prot5439 (2010)
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商品

Genomic DNA Purification and Assessment

Simple DNA/RNA purification methods aid genome analysis from various sources, enhancing research efficiency.

基因组DNA样品纯化和质量评估

简单的DNA和RNA纯法方法大幅推动了基因组和基因表达的分析和鉴定。人们需要快速、方便地从多种细胞来源分离DNA和RNA，包括哺乳动物、植物和细菌培养物来源的细胞和组织。

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

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