FastStart™ 通用SYBR® Green预混液(ROX)

Watch these videos to learn how real time or quantitative PCR (qPCR) works and the benefits of both the SYBR Green-based and probe-based methods of qPCR assay.

The purpose of Hot Start PCR is to inhibit the PCR reaction in order to reduce nonspecific amplification, prevent the formation of primer dimers, and increase product yields.

热启动PCR的目的在于抑制PCR反应，从而减少非特异性扩增、防止引物二聚体形成并提高产物产量。

Polymerase chain reaction (PCR) is a technique for amplifying nucleic acid molecules and is commonly used in many applications, including RT-PCR, hot start PCR, end point PCR and more.

PCR是分子生物学的核心技术，功能强大。可在体外高效快速地对不同来源的特定DNA或RNA序列进行酶扩增。

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RT-qPCR detects specific targets with applications in gene expression and pathogen detection.