D7290
Deoxyribonucleic acid from herring sperm
single-stranded for hybridization
Synonym(s):
DNA
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50 EA
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CN¥4,855.76
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50 EA
CN¥4,855.76
About This Item
CAS Number:
EC Number:
MDL number:
UNSPSC Code:
41106310
eCl@ss:
32160414
NACRES:
NA.52
CN¥4,855.76
Please contact Customer Service for Availability
Recommended Products
biological source
(herring sperm)
Quality Level
grade
Molecular Biology
form
solution
shipped in
dry ice
storage temp.
−20°C
1 of 4
This Item | SAB1411370 | SAB1406563 | SAB1409776 |
|---|---|---|---|
| biological source rabbit | biological source rabbit | biological source mouse | biological source mouse |
| Quality Level 100 | Quality Level 100 | Quality Level - | Quality Level 100 |
| technique(s) immunohistochemistry: 1:50-1:200, western blot: 1:500-1:2000 | technique(s) western blot: 1 μg/mL | technique(s) indirect immunofluorescence: suitable, western blot: 1 μg/mL | technique(s) immunoprecipitation (IP): suitable, indirect ELISA: suitable, western blot: 1-5 μg/mL |
| shipped in wet ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
| species reactivity mouse, rat, human | species reactivity human | species reactivity human | species reactivity human |
| clone polyclonal | clone polyclonal | clone polyclonal | clone 2G12, monoclonal |
General description
Suitable for use as a blocking agent in Southern hybridizations.
Application
Deoxyribonucleic acid from herring sperm is suitable for use as a blocking agent in Southern hybridizations. It was used in preparation of brain sections of mice for BrdU immunohistochemistry.[1] It was also used for hybridization reaction of dot blot membranes.[2]
In order to decrease any non-specific hybridization of the probe to a substrate, blocking agents must be used. Generally, a combination of blocking reagent, detergent, and denatured, fragmented DNA is used to accomplish this. Sigma offers sonicated, denatured DNA from a variety of species for use as a blocking agent in Northern and Southern blotting and other nucleic acid hybridization techniques.
Many factors contribute to the signal-to-noise ratio in nucleic acid hybridizations. These factors include the presence of solvent (formamide), hybridization temperature, length of hybridization, volume of hybridization solution, degree and method of agitation, use of blocking reagents, concentration and specific activity of the probe, use of molecular agents to increase the rate of nucleic acid reassociation, and the degree of stringency used during the washing of the membrane.
In order to decrease any non-specific hybridization of the probe to a substrate, blocking agents must be used. Generally, a combination of blocking reagent, detergent, and denatured, fragmented DNA is used to accomplish this. Sigma offers sonicated, denatured DNA from a variety of species for use as a blocking agent in Northern and Southern blotting and other nucleic acid hybridization techniques.
In order to decrease any non-specific hybridization of the probe to a substrate, blocking agents must be used. Generally, a combination of blocking reagent, detergent, and denatured, fragmented DNA is used to accomplish this. Sigma offers sonicated, denatured DNA from a variety of species for use as a blocking agent in Northern and Southern blotting and other nucleic acid hybridization techniques.
Preparation Note
This DNA is phenol-chloroform extracted, ethanol precipitated, and sonicated to produce single-stranded fragments which comigrate with the 587 and 831 base pair marker fragments.
Other Notes
DNA in solution will reanneal on standing at room temperature so it is recommended to boil the solution for 10 minutes and then cool on ice for at least 5 minutes prior to use.
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Product No.
Description
Pricing
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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