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Key Documents

S2015

Sigma-Aldrich

SSPE Buffer 20× Concentrate

for Northern and Southern blotting, solution

Synonym(s):

20X SSPE Buffer, SSPE hybridization buffer, Saline-sodium phosphate-EDTA buffer

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1 L
¥951.34

¥951.34

Available to ship on2025年4月25日Details

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1 L
¥951.34

About This Item

UNSPSC Code:
41105319
NACRES:
NA.25

¥951.34

Available to ship on2025年4月25日Details

Request a Bulk Order

grade

Molecular Biology

Quality Level

200

sterility

0.2 μm filtered

form

solution

foreign activity

DNAse, none detected
Endonuclease, none detected
Exonuclease, none detected
NICKase, none detected
RNAse, none detected

storage temp.

room temp

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S6639S8140SRE0068
SSPE 缓冲液 20× 浓缩液 for Northern and Southern blotting, solution

S2015

SSPE 缓冲液 20× 浓缩液

SSC 20x 浓缩缓冲液 for Northern and Southern blotting, solution

S6639

SSC 20x 浓缩缓冲液

SSPE Buffer for Northern and Southern blotting, powder blend

S8140

SSPE Buffer

Sigma-Aldrich

SRE0068

SSC缓冲液，20倍浓缩液

grade

for molecular biology

grade

for molecular biology

grade

for molecular biology

grade

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

500

form

solution

form

solution

form

powder blend

form

liquid

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

storage temp.

2-8°C

foreign activity

DNAse, none detected, Exonuclease, none detected, RNAse, none detected, Endonuclease, none detected, NICKase, none detected

foreign activity

DNAse, none detected, Endonuclease, none detected, Exonuclease, none detected, NICKase, none detected, RNAse, none detected

foreign activity

DNAse, none detected, Endonuclease, none detected, Exonuclease, none detected, NICKase, none detected, RNAse, none detected

foreign activity

-

sterility

0.2 μm filtered

sterility

0.2 μm filtered

sterility

-

sterility

-

General description

SSPE Buffer 20x liquid is a standard reagent in Southern and Northern hybridization procedures. This liquid can be used straight or diluted as needed.

Application

Suitable for:
  • Hybridization buffer for Northern and Southern blots[1]
  • Washing buffer for microarrays[2]

Features and Benefits

  • Convenient ready-to-use liquid
  • Multi-purpose use in hybridizations

Preparation Note

SSPE Buffer 20x contains 0.02M EDTA and 2.98M NaCl in 0.2M phosphate buffer (pH 7.4).

related product

Product No.
Description
Pricing

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

  • Specification Sheet

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    Certificates of Analysis (COA)

    Lot/Batch Number
    0000446238
    0000446238
    0000406274
    0000405184
    0000395525

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    Protocols

    Northern & Southern Blot Protocols

    Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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