S0902
SSC Buffer
for Northern and Southern blotting, powder blend
Synonym(s):
Saline-sodium citrate buffer
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About This Item
UNSPSC Code:
41105319
NACRES:
NA.25
Recommended Products
Quality Level
form
powder blend
pH
6.8-7.2 (20-25 °C, 263 mg/L in water)
solubility
water: 263 mg/mL, clear, colorless
foreign activity
DNAse, none detected
Endonuclease, none detected
Exonuclease, none detected
NICKase, none detected
RNAse, none detected
storage temp.
room temp
General description
SSC Buffer 20x Powder is a standard reagent in Southern and Northern blotting procedures. This concentrated powder should be reconstituted in molecular biology grade water to the indicated final volume before use (final solution is a 20x). The 20x concentrate can be used straight or diluted to make SSC working solutions with concentrations as low as 0.5x.
Application
Saline-sodium citrate (SSC) Buffer has been used as a washing buffer in microarray technique. It has also been used as a constituent of fluorescence in situ hybridization (FISH) buffer.
Suitable for:
- Preparing nucleic acids for hybridization
- Hybridization buffer for Northern and Southern blots
- Washing buffer for Northern and Southern blots
Features and Benefits
- Multi-purpose use in hybridizations
- Easily reconstituted and diluted to any concentration needed
Components
Final SSC Buffer 20x Concentrate contains 0.3 M sodium citrate in 3M NaCl.
related product
Product No.
Description
Pricing
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Autophagy, 12(2), 273-286 (2016)
M Böttger et al.
Nucleic acids research, 9(20), 5253-5268 (1981-10-24)
Complexes of histones H1 with superhelical SV40 DNA obtained by direct mixing were studied in 0.1 SSC buffer corresponding to 0.02 M Na+. Depending on the molar input ratio H1/DNA three classes of sedimenting species were observed: (1) a component
Aline V Probst et al.
Developmental cell, 19(4), 625-638 (2010-10-19)
At the time of fertilization, the paternal genome lacks the typical configuration and marks characteristic of pericentric heterochromatin. It is thus essential to understand the dynamics of this region during early development, its importance during that time period and how
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Nature genetics, 43(3), 220-227 (2011-02-15)
HP1 enrichment at pericentric heterochromatin is considered important for centromere function. Although HP1 binding to H3K9me3 can explain its accumulation at pericentric heterochromatin, how it is initially targeted there remains unclear. Here, in mouse cells, we reveal the presence of
Protocols
Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.
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