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T9285

Sigma-Aldrich

Tris-EDTA buffer solution

100X concentrate for Northern and Southern blotting

Synonym(s):

Tris-EDTA wash buffer, TE buffer solution

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10 ML
¥5,623.54

¥5,623.54

Available to ship on2025年4月07日Details

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10 ML
¥5,623.54

About This Item

MDL number:
MFCD00236359
UNSPSC Code:
41105319
PubChem Substance ID:
329826904
NACRES:
NA.25

¥5,623.54

Available to ship on2025年4月07日Details

Request a Bulk Order

grade

Molecular Biology
for molecular biology

Quality Level

200

sterility

0.2 μm filtered

form

liquid

pH

7.8-8.2 (1 × in water)

application(s)

sample preparation

foreign activity

DNase, RNase, none detected

storage temp.

room temp

SMILES string

NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O

InChI

1S/C10H16N2O8.C4H11NO3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2

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Silver, dispersion nanoparticle, 30-35 wt. % in triethylene glycol monoethyl ether, spec. resistivity 11 μΩ-cm, for printing on plastic films

736465

Silver, dispersion

Silver, dispersion nanoparticle, 40-50 wt. %, spec. resistivity < 0.001 Ω-cm, for printing on ITO and glass

736511

Silver, dispersion

Silver nanopowder, <100 nm particle size, contains PVP as dispersant, 99.5% trace metals basis

576832

Silver

Silver, dispersion nanoparticles, 10 nm particle size (TEM), 0.02 mg/mL in aqueous buffer, contains sodium citrate as stabilizer

730785

Silver, dispersion

form

dispersion, nanoparticle

form

dispersion, nanoparticle

form

nanopowder

form

nanoparticles

description

surface tension 35 - 40 dyn/cm

description

surface tension 40 - 70 mN m-1

description

-

description

-

concentration

30-35 wt. % in triethylene glycol monoethyl ether

concentration

40-50 wt. %

concentration

-

concentration

0.02 mg/mL in aqueous buffer

refractive index

n20/D 1.333

refractive index

n20/D 1.333

refractive index

-

refractive index

n20/D 1.333

viscosity

10-18 cP

viscosity

900-1600 mPa.s ((25°C))

viscosity

-

viscosity

-

General description

Tris EDTA buffer is commonly used in molecular biology for reconstitution and storage of many precipitated biomolecules, including DNA and RNA. Tris-HCl is a commonly used buffer and EDTA is a metal chelating agent. TE buffer solubilizes nucleic acids while protecting the molecules from degradation. It is used in nucleic acid isolation, which may be done prior to nucleic acid gel electrophoresis and Northern or Southern blot hybridization.

Application

TE buffer has been used for:
  • isolate, resuspend, or store DNA and RNA for PCR or qPCR[1][2]
  • gel electrophoresis, including Northern and Southern blotting[3]
  • bacterial cells, for AmpC disk test, to measure β-lactamases released from the cells[4]
  • antigen retrieval for immunohistochemistry[3]

Components

TE Buffer contains 1M Tris-HCl (pH approximately 8.0), containing 0.1M EDTA.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

  • Specification Sheet

    • Choose from one of the most recent versions:

    Certificates of Analysis (COA)

    Lot/Batch Number
    SLCK0421
    SLCJ8255
    0000391230
    0000390095
    0000386778

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    If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

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    1. Which document(s) contains shelf-life or expiration date information for a given product?

      If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

    2. How do I get lot-specific information or a Certificate of Analysis?

      The lot specific COA document can be found by entering the lot number above under the "Documents" section.

    3. How do I find price and availability?

      There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

    4. What is the Department of Transportation shipping information for this product?

      Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

    5. What are the final concentrations of Tris and EDTA when Product T9285 is diluted to 1:100 to make it 1X?

      When diluted 1:100, the solution is 10mM TRIS with 1mM EDTA.

    6. What is the pH and color of Product T9285, Tris-EDTA buffer solution?

      The pH and color information is available on the specification sheet as well as lot specific Certificate of Analysis.

    7. What is the shelf life of Product T9285, Tris-EDTA buffer solution?

      This buffer solution has a recommended retest date of three years. This information is available on the Specification Sheet, and on the lot-specific Certificates of Analysis.

    8. What does 'for molecular biology' mean?

      The phrase `for molecular biology' means that all lots of this buffer have been tested for DNase and RNase activity, and that no activity was detected.

    9. My question is not addressed here, how can I contact Technical Service for assistance?

      Ask a Scientist here.

    Marika Bogdani et al.
    Scientific reports, 7(1), 17231-17231 (2017-12-10)
    Cystic fibrosis (CF)-related diabetes (CFRD) is thought to result from beta-cell injury due in part to pancreas exocrine damage and lipofibrosis. CFRD pancreata exhibit reduced islet density and altered cellular composition. To investigate a possible etiology, we tested the hypothesis
    Virtual Fluorescence Color Channels by Selective Photobleaching in Digital PCR Applied to the Quantification of KRAS Point Mutations
    Schlenker F et al.
    Analytical Chemistry, 93, 10538-10545 (2021)
    Sarah Bøje et al.
    Immunology and cell biology, 94(2), 185-195 (2015-08-14)
    Chlamydia is the most widespread sexually transmitted bacterial disease and a prophylactic vaccine is highly needed. Ideally, this vaccine is required to induce a combined response of Th1 cell-mediated immune (CMI) response in concert with neutralizing antibodies. Using a novel
    Jennifer A Black et al.
    Journal of clinical microbiology, 43(7), 3110-3113 (2005-07-08)
    Although plasmid-mediated AmpC beta-lactamases were first reported in the late 1980s, many infectious disease personnel remain unaware of their clinical importance. These enzymes are typically produced by isolates of Escherichia coli, Klebsiella spp., Proteus mirabilis, and Salmonella spp. and are
    AmpC disk test for detection of plasmid-mediated AmpC beta-lactamases in Enterobacteriaceae lacking chromosomal AmpC beta-lactamases.
    Black JA, et al.
    Journal of Clinical Microbiology, 43, 3110-3113 (2005)
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