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T9285

Sigma-Aldrich

Tris-EDTA buffer solution

100X concentrate for Northern and Southern blotting

Synonym(s):

Tris-EDTA wash buffer, TE buffer solution

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100 ML
CN¥474.77

CN¥474.77

Available to ship onApril 14, 2025Details

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100 ML
CN¥474.77

About This Item

MDL number:
MFCD00236359
UNSPSC Code:
41105319
PubChem Substance ID:
329826904
NACRES:
NA.25

CN¥474.77

Available to ship onApril 14, 2025Details

Request a Bulk Order

grade

Molecular Biology

Quality Level

200

sterility

0.2 μm filtered

form

liquid

pH

7.8-8.2 (1 × in water)

application(s)

sample preparation

foreign activity

DNase, RNase, none detected

storage temp.

room temp

SMILES string

NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O

InChI

1S/C10H16N2O8.C4H11NO3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2

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1 of 4

This Item
93283T4415T9650
Tris-EDTA buffer solution 100X concentrate for Northern and Southern blotting

T9285

Tris-EDTA buffer solution

Tris-EDTA buffer solution BioUltra, pH 8.0

93283

Tris-EDTA buffer solution

Tris-Borate-EDTA buffer BioReagent, suitable for electrophoresis, 10× concentrate

T4415

Tris-Borate-EDTA buffer

Tris Acetate-EDTA buffer 10× concentrate, BioReagent, non-sterile; 0.2 μm filtered

T9650

Tris Acetate-EDTA buffer

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

application(s)

sample preparation

application(s)

sample preparation

application(s)

-

application(s)

-

form

liquid

form

liquid

form

solution

form

solution

pH

7.8-8.2 (1 × in water)

pH

8.0±0.2 (25 °C)

pH

-

pH

-

storage temp.

room temp

storage temp.

-

storage temp.

-

storage temp.

-

General description

Tris EDTA buffer is commonly used in molecular biology for reconstitution and storage of many precipitated biomolecules, including DNA and RNA. Tris-HCl is a commonly used buffer and EDTA is a metal chelating agent. TE buffer solubilizes nucleic acids while protecting the molecules from degradation. It is used in nucleic acid isolation, which may be done prior to nucleic acid gel electrophoresis and Northern or Southern blot hybridization.

Application

TE buffer has been used for:
  • isolate, resuspend, or store DNA and RNA for PCR or qPCR[1][2]
  • gel electrophoresis, including Northern and Southern blotting[3]
  • bacterial cells, for AmpC disk test, to measure β-lactamases released from the cells[4]
  • antigen retrieval for immunohistochemistry[3]

Preparation Note

TE Buffer contains 1M Tris-HCl (pH approximately 8.0), containing 0.1M EDTA.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

  • Specification Sheet

    • Choose from one of the most recent versions:

    Certificates of Analysis (COA)

    Lot/Batch Number
    0000443473
    0000443473
    SLCK0421
    SLCJ8255
    0000391230

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    If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

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    1. Which document(s) contains shelf-life or expiration date information for a given product?

      If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

    2. How do I get lot-specific information or a Certificate of Analysis?

      The lot specific COA document can be found by entering the lot number above under the "Documents" section.

    3. How do I find price and availability?

      There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

    4. What is the Department of Transportation shipping information for this product?

      Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

    5. What are the final concentrations of Tris and EDTA when Product T9285 is diluted to 1:100 to make it 1X?

      When diluted 1:100, the solution is 10mM TRIS with 1mM EDTA.

    6. What is the pH and color of Product T9285, Tris-EDTA buffer solution?

      The pH and color information is available on the specification sheet as well as lot specific Certificate of Analysis.

    7. What is the shelf life of Product T9285, Tris-EDTA buffer solution?

      This buffer solution has a recommended retest date of three years. This information is available on the Specification Sheet, and on the lot-specific Certificates of Analysis.

    8. What does 'for molecular biology' mean?

      The phrase `for molecular biology' means that all lots of this buffer have been tested for DNase and RNase activity, and that no activity was detected.

    9. My question is not addressed here, how can I contact Technical Service for assistance?

      Ask a Scientist here.

    Sudheesh N et al.
    Ticks and tick-borne diseases, 11(3), 101381-101381 (2020-02-06)
    Kyasanur Forest disease virus (KFDV) and Alkhurma hemorrhagic fever virus (AHFV) are enveloped, positive-stranded RNA viruses of clinical importance with complex enzootic life cycles involving hematophagous ticks which feed on small and large mammals. Humans and monkeys are dead-end hosts
    Kashif Asghar et al.
    Experimental and therapeutic medicine, 9(3), 901-904 (2015-02-11)
    Indoleamine 2,3-dioxygenase (IDO) is an immunoregulatory enzyme. It plays a key role in various malignancies, infection and autoimmune diseases. IDO induces immunosuppression through the depletion of tryptophan and its downstream metabolites. Hepatitis C virus (HCV) has infected more than 12
    AmpC disk test for detection of plasmid-mediated AmpC beta-lactamases in Enterobacteriaceae lacking chromosomal AmpC beta-lactamases.
    Black JA, et al.
    Journal of Clinical Microbiology, 43, 3110-3113 (2005)
    Shiraz Tyebji et al.
    Cell reports, 31(4), 107573-107573 (2020-04-30)
    Germline epigenetic factors influence transgenerational inheritance of behavioral traits upon changes in experience and environment. Immune activation due to infection can also modulate brain function, but whether this experience can be passed down to offspring remains unknown. Here, we show
    Brian O'Farrell et al.
    PloS one, 7(10), e48022-e48022 (2012-11-13)
    Microbial genotyping increasingly deals with large numbers of samples, and data are commonly evaluated by unstructured approaches, such as spread-sheets. The efficiency, reliability and throughput of genotyping would benefit from the automation of manual manipulations within the context of sophisticated
    View All Related Papers

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