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Key Documents

Safety Information

B6429

Sigma-Aldrich

Casein Blocking Buffer 10x

for Northern and Southern blotting, solution

Synonym(s):

10x Blocking Buffer

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1 EA
¥10,654.94

¥10,654.94

Available to ship on2025年4月14日Details

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1 EA
¥10,654.94

About This Item

UNSPSC Code:
12161700
NACRES:
NA.54

¥10,654.94

Available to ship on2025年4月14日Details

grade

Molecular Biology

Quality Level

200

form

solution

technique(s)

Southern blotting: suitable

storage temp.

room temp

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This Item
582711U53827U50630U
Ascentis® Express C18, 2.7 μm HPLC Column 2.7 μm particle size, L × I.D. 15 cm × 4.6 mm

53829U

Ascentis® Express C18, 2.7 μm HPLC Column

Ascentis® Express C18, 2.7 μm HPLC Column 2.7 μm particle size, L × I.D. 5 cm × 1 mm

582711U

Ascentis® Express C18, 2.7 μm HPLC Column

Ascentis® Express C18, 2.7 μm HPLC Column 2.7 μm particle size, L × I.D. 10 cm × 4.6 mm

53827U

Ascentis® Express C18, 2.7 μm HPLC Column

Ascentis® Express C18, 2.7 μm HPLC Column particle size, std dev 2.7 μm, L × I.D. 10 cm × 1.5 mm

50630U

Ascentis® Express C18, 2.7 μm HPLC Column

particle size

2.7 μm

particle size

2.7 μm

particle size

2.7 μm

particle size

std dev 2.7 μm

matrix

Fused-Core particle platform, superficially porous particle

matrix

Fused-Core particle platform, superficially porous particle

matrix

Fused-Core particle platform, superficially porous particle

matrix

Fused-Core particle platform

separation technique

reversed phase

separation technique

reversed phase

separation technique

reversed phase

separation technique

-

agency

suitable for USP L1

agency

suitable for USP L1

agency

suitable for USP L1

agency

suitable for USP L1

technique(s)

HPLC: suitable, UHPLC-MS: suitable, LC/MS: suitable, UHPLC: suitable

technique(s)

HPLC: suitable, LC/MS: suitable, UHPLC-MS: suitable, UHPLC: suitable

technique(s)

HPLC: suitable, LC/MS: suitable

technique(s)

-

General description

Casein is a phosphoprotein found in milk. It is classified into four subtypes, including αS1-, αS2-, β-, and κ-caseins.[1]
Sigma’s 10x Blocking Buffer is a casein-based blocking agent routinely used to reduce non-specific binding of detection reagents[1] to the Northern and Southern Blot membranes.

Application

Casein Blocking Buffer 10x has been used in:

  • western blotting[2][3][4]
  • enzyme linked immunosorbent assay (ELISA)[5]
  • immunohistochemistry[6]

Features and Benefits

  • Better blocking performance than dried milk or BSA
  • Easy-to-use concentrate
  • Compatible with neutral and positively-charged membranes
  • Compatible with chemiluminescent and colorimetric detection assays

Preparation Note

Dilute 10x Blocking Buffer with molecular biology grade water before use.

Pictograms

Exclamation markEnvironment
GHS07,GHS09

Signal Word

Warning

Hazard Statements

H315,H317,H319,H411

Hazard Classifications

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

监管及禁止进口产品

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Certificates of Analysis (COA)

Lot/Batch Number
0000386787
0000377173
0000368122
0000377173
0000368122

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Mihai D Niculescu et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 20(1), 43-49 (2006-01-06)
The availability of choline during critical periods of fetal development alters hippocampal development and affects memory function throughout life. Choline deficiency during fetal development reduces proliferation and migration of neuronal precursor cells in the mouse fetal hippocampus and these changes
Choline availability modulates human neuroblastoma cell proliferation and alters the methylation of the promoter region of the cyclin-dependent kinase inhibitor 3 gene
Niculescu MD, et al.
Journal of Neurochemistry, 89(5), 1252-1259 (2004)
Tanja Kalic et al.
The journal of allergy and clinical immunology. In practice, 8(9), 3084-3092 (2020-05-12)
Fish collagen is widely used in medicine, cosmetics, and the food industry. However, its clinical relevance as an allergen is not fully appreciated. This is likely due to collagen insolubility in neutral aqueous solutions, leading to low abundance in commercially
M B Engelund et al.
American journal of physiology. Regulatory, integrative and comparative physiology, 302(2), R300-R311 (2011-10-07)
Claudins are the major determinants of paracellular epithelial permeability in multicellular organisms. In Atlantic salmon (Salmo salar L.), we previously found that mRNA expression of the abundant gill-specific claudin 30 decreases during seawater (SW) acclimation, suggesting that this claudin is
Malene Møller Jørgensen et al.
Polymers, 13(14) (2021-07-25)
Extracellular vesicles (EVs) are promising biomarkers for several diseases, however, no simple and robust methods exist to characterize EVs in a clinical setting. The EV Array analysis is based on a protein microarray platform, where antibodies are printed onto a
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Protocols

Casein Blocking Buffer Protocol

Protocol for Casein Blocking Buffer in Southern and Western blotting includes troubleshooting for optimizing signal.

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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