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Merck
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Key Documents

15356

Sigma-Aldrich

Blotting-Nylon 66 membranes, type B, positive

for Northern and Southern blotting

Synonym(s):

Nylon 66-Blotting membranes, type B

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5 μG
CN¥1,612.10
4 X 5 μG
CN¥3,069.85

CN¥1,612.10

Available to ship onApril 10, 2025Details

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5 μG
CN¥1,612.10
4 X 5 μG
CN¥3,069.85

About This Item

UNSPSC Code:
41105339
NACRES:
NA.25

CN¥1,612.10

Available to ship onApril 10, 2025Details

material

Nylon

grade

Molecular Biology
for molecular biology

form

roll

surface coverage

surface (quaternary ammonium groups) ≥99%

pore size

0.45 μm pore size

storage temp.

room temp

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Granulocyte-Macrophage Colony-Stimulating Factor human GM-CSF, recombinant, expressed in E. coli, suitable for cell culture

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Granulocyte-Macrophage Colony-Stimulating Factor human

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G0282

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Macrophage Colony-Stimulating Factor human M-CSF, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

M6518

Macrophage Colony-Stimulating Factor human

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

biological source

human

biological source

human

biological source

mouse

biological source

human

assay

≥98% (SDS-PAGE)

assay

≥98% (SDS-PAGE)

assay

≥97% (SDS-PAGE)

assay

≥98% (SDS-PAGE and HPLC)

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

form

lyophilized powder

form

lyophilized

form

lyophilized powder

form

lyophilized powder

General description

Blotting nylon provided on a roll is a standard item used in transfer of protein or nucleic acids during Western, Southern and Northern procedures. This membrane is positively charged nylon cast on a polyester support. It comes as a roll sized 0.2m x 3m, and can be cut to any size.

Application

Suitable for:
Protein transfer
Radioactive detection of blotted proteins and nucleic acids
Sensitive dot and slot blots of nucleic acids
for high-sensitive nucleic acid blotting, dot and lot blots. Particularly suitable for radioactive detection. Suitable for protein transfer and immunoblotting.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves
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    K Pfeifer et al.
    Journal of cell science, 106 ( Pt 2), 545-553 (1993-10-01)
    Ubiquitination of proteins is a critical step in the controlled degradation process of many polypeptides. Here we show that sponges, the simplest multicellular group of eukaryotic organisms, are also equipped with the ubiquitin pathway. The polyubiquitin cDNA was isolated and
    Sambrook, J., et al.
    Molecular Cloning: A Laboratory Manual, 9-9 (1989)
    Gerardas Dambrauskas et al.
    Journal of experimental botany, 54(383), 699-706 (2003-01-30)
    Very little is known about the expression patterns in plants of genes that encode proteins involved in the initiation of DNA replication. Partial cDNA sequences that encode Cdc6 and Mcm3 in tobacco have been isolated. The sequences were used as

    Protocols

    Northern & Southern Blot Protocols

    Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

    Contact Technical Service