P1415
Prehybridization Solution
for Northern and Southern blotting, liquid (2x concentrate)
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About This Item
UNSPSC Code:
12161700
NACRES:
NA.25
Quality Level
100
200
form
liquid (2x concentrate)
foreign activity
DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected
storage temp.
−20°C
Related Categories
General description
Sigma′s Prehybridization Solution is a 2x ready-to-use buffer for Northern and Southern blot protocols.
Application
Suitable for use as a pre-hybridization buffer in Northern and Southern blot protocols.
Components
Sigma′s 2X Prehybridization Solution contains 200 μg/mL DNA in 10x SSC and 2X Denhardt′s solutions:
- 200 μg/mL DNA
- 2mg Bovine Serum Albumin (BSA)
- 2mg Ficoll
- 2mg Polyvinylpyrrolidone (PVP)
- 0.15 M sodium citrate
- 1.5M NaCl
related product
Product No.
Description
Pricing
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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Human reproduction (Oxford, England), 11(12), 2629-2634 (1996-12-01)
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Elizabeth S Colombo et al.
Investigative ophthalmology & visual science, 48(4), 1793-1800 (2007-03-29)
The purpose of this study was to determine the role of hepatocyte growth factor (HGF) and c-Met in the initiation and development of retinal neovascularization and to determine whether inhibition of this system can suppress the extent of angiogenesis in
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 9-9 (1989)
Andrew M Pilling et al.
Toxicologic pathology, 35(3), 348-355 (2007-04-25)
Spontaneous follicular and C-cell tumors of the thyroid gland in the Han Wistar rat were examined using two morphologic procedures. Firstly, in situ hybridization (ISH) was used to localize thyroglobulin (TG) and calcitonin (CT) mRNAs. Secondly, the proteins for these
Jianmin Su et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 40(39), 7421-7435 (2020-08-28)
GABAergic interneurons represent a heterogenous group of cell types in neocortex that can be clustered based on developmental origin, morphology, physiology, and connectivity. Two abundant populations of cortical GABAergic interneurons include the low-threshold, somatostatin (SST)-expressing cells and the fast-spiking, parvalbumin
Protocols
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