N1531
Denaturation Solution
for Northern and Southern blotting
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Synonym(s):
Denaturation Solution For Blotting Technique, Denaturation Solution for Neutral Southern Transfer
UNSPSC Code:
12352200
NACRES:
NA.25
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General description
Sigma′s denaturing solution is a strong base that denatures double-stranded DNA so that it can be efficiently transferred to nylon membrane and subsequently hybridized to labeled probe. When used with a depurination solution, treatment results in the hydrolysis of the phosphodiester backbone at the sites of depurination, allowing for more efficient transfer of large DNA in neutral Southern transfers.
Application
Denaturation Solution is suitable for denaturing dsDNA before the transfer to membranes during Northern and Southern blotting procedures.
Components
The denaturing solutions is composed of 0.5N NaOH, and 1.5M NaCl.
Other Notes
0.5 N NaOH, 1.5 M NaCl
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Product No.
Description
Pricing
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1B
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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José Manuel Pérez-Pérez et al.
Plant physiology, 134(1), 101-117 (2004-01-20)
The dwarf ucu (ultracurvata) mutants of Arabidopsis display vegetative leaves that are spirally rolled downwards and show reduced expansion along the longitudinal axis. We have previously determined that the UCU1 gene encodes a SHAGGY/GSK3-like kinase that participates in the signaling
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 9-9 (1989)
Ausubel, F.M., et al.
Short Protocols in Molecular Biology (1995)
Protocols
Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.
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