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M5755

Sigma-Aldrich

MOPS-EDTA-Sodium Acetate (MESA)

powder for RNA electrophoresis buffers

Synonym(s):

MESA Buffer

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Select a Size

10 ML
¥2,336.73
10 X 1 ML
¥2,921.95
50 ML
¥10,870.71

¥2,336.73

Available to ship on2025年4月17日Details

Request a Bulk Order
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10 ML
¥2,336.73
10 X 1 ML
¥2,921.95
50 ML
¥10,870.71

About This Item

UNSPSC Code:
12161700
NACRES:
NA.25

¥2,336.73

Available to ship on2025年4月17日Details

Request a Bulk Order

grade

Molecular Biology

Quality Level

200

product line

BioReagent

form

powder

technique(s)

electrophoresis: suitable

pH

6.8-7.2(reconstituted per directions)

solubility

H2O: soluble, faintly yellow

foreign activity

DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected

storage temp.

room temp

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This Item
91707T6508618729
Trifluoroacetic acid eluent additive for LC-MS, LiChropur™, ≥99.0% (GC)

80457

Trifluoroacetic acid

Trifluoroacetic acid puriss. p.a., suitable for HPLC, ≥99.0% (GC)

91707

Trifluoroacetic acid

Trifluoroacetic acid ReagentPlus®, 99%

T6508

Trifluoroacetic acid

Trifluoroacetic acid

618729

Trifluoroacetic acid

assay

≥99.0% (GC)

assay

≥99.0% (GC)

assay

99%

assay

-

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

100

form

liquid

form

liquid

form

liquid

form

-

refractive index

n20/D 1.3 (lit.)

refractive index

n20/D 1.3 (lit.)

refractive index

n20/D 1.3 (lit.)

refractive index

-

density

1.489 g/mL at 20 °C (lit.)

density

1.489 g/mL at 20 °C (lit.)

density

1.489 g/mL at 20 °C (lit.)

density

-

General description

MOPS – EDTA – Sodium Acetate (MESA) is the most commonly used running buffer for RNA electrophoresis prior to Northern blotting procedures. Applied voltage of 5 V/cm is recommended for maximum resolution.

Application

Suitable for use when making formaldehyde-agarose gels and associated running buffer for RNA electrophoresis.

Preparation Note

1x MESA buffer contains:
  • 40 mM MOPS
  • 10 mM sodium acetate
  • 1 mM EDTA (pH 8.3)

Reconstitution

Dissolve entire contents of bottle in a final volume of 1L molecular biology grade water. This produces a 10x MESA stock solution that can be further diluted as needed.

related product

Product No.
Description
Pricing

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves
  • Specification Sheet

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    Certificates of Analysis (COA)

    Lot/Batch Number
    0000383887
    0000257463
    0000344083
    0000257463
    0000344083

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    Kathryn M Jones et al.
    Journal of bacteriology, 184(9), 2491-2499 (2002-04-12)
    Two operons have been cloned from Anabaena sp. strain PCC 7120 DNA, each of which encodes the three core subunits of distinct mitochondrial-type cytochrome c oxidases. The two operons are only 72 to 85% similar to one another at the
    Xueyu Xiang et al.
    Developmental biology, 275(2), 343-355 (2004-10-27)
    Recently, we cloned and sequenced the cDNA of allurin, a sperm chemoattractant isolated from the jelly of Xenopus laevis eggs [Proc. Natl. Acad. Sci. U.S.A. 78 (2001) 11205]. In this report, we demonstrate that allurin mRNA is expressed almost exclusively
    Molecular techniques for detection of gene expression in neuroimmunology.
    Cowan, E.P., and Dhib-Jalbut, S.S.
    Methods in Neuroscience, 24, 41-41 (1995)
    Sambrook, J., et al.
    Molecular Cloning: A Laboratory Manual, 7-7 (1989)
    Electrophoresis in agarose and acrylamide gels.
    R C Ogden et al.
    Methods in enzymology, 152, 61-87 (1987-01-01)
    View All Related Papers

    Protocols

    TAE and TBE Running Buffers Recipe & Video

    TAE and TBE are both used as running buffers for nucleic acid electrophoresis but have some important differences. Review our recipes and video to give your application the best chance of success.

    Northern & Southern Blot Protocols

    Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

    Contact Technical Service