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D3160

Sigma-Aldrich

Deoxyribonucleic acid from human placenta

buffered aqueous solution, sexed, male

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Synonym(s):
DNA
CAS Number:
9007-49-2
MDL number:
MFCD00130921
UNSPSC Code:
12352200

grade

for molecular biology

form

buffered aqueous solution

shipped in

dry ice

storage temp.

−20°C

InChI

1S/C15H31N3O13P2/c16-13-1-7(20)11(28-13)5-25-32(21,22)31-9-3-15(18)29-12(9)6-26-33(23,24)30-8-2-14(17)27-10(8)4-19/h7-15,19-20H,1-6,16-18H2,(H,21,22)(H,23,24)

InChI key

AWBASQCACWFTGD-UHFFFAOYSA-N

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General description

Human placental DNA is isolated from donor placenta, but will contain some maternal DNA. The DNA fragments are sonicated to produce fragments of consistent size.

Application

Deoxyribonucleic acid from human placenta is used as blocking agent in Southern hybridizations. It was used to compare the efficiency of DNA quantification methods.
For use in Southern hybridizations.

Components

DNA is supplied in a solution of 10mM Tris-HCl (pH 8.4), with 1mM EDTA.

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WGK

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Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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Karsten Nielsen et al.
Forensic science international. Genetics, 2(3), 226-230 (2008-12-17)
Six commercial preparations of human genomic DNA were quantified using five quantification methods: UV spectrometry, SYBR-Green dye staining, slot blot hybridization with the probe D17Z1, Quantifiler Human DNA Quantification kit and RB1 rt-PCR. All methods measured higher DNA concentrations than
Zachary D Smith et al.
Nature, 511(7511), 611-615 (2014-08-01)
In mammals, cytosine methylation is predominantly restricted to CpG dinucleotides and stably distributed across the genome, with local, cell-type-specific regulation directed by DNA binding factors. This comparatively static landscape is in marked contrast with the events of fertilization, during which
Manolis Kellis et al.
Proceedings of the National Academy of Sciences of the United States of America, 111(17), 6131-6138 (2014-04-23)
With the completion of the human genome sequence, attention turned to identifying and annotating its functional DNA elements. As a complement to genetic and comparative genomics approaches, the Encyclopedia of DNA Elements Project was launched to contribute maps of RNA
Maarten Holkers et al.
Nature methods, 11(10), 1051-1057 (2014-08-26)
Engineered sequence-specific nucleases and donor DNA templates can be customized to edit mammalian genomes via the homologous recombination (HR) pathway. Here we report that the nature of the donor DNA greatly affects the specificity and accuracy of the editing process
Xin Cai et al.
Molecular cell, 54(2), 289-296 (2014-04-29)
The innate immune system deploys a variety of sensors to detect signs of infection. Nucleic acids represent a major class of pathogen signatures that can trigger robust immune responses. The presence of DNA in the cytoplasm of mammalian cells is
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