D7656
Deoxyribonucleic acid, single stranded from salmon testes
For hybridization
Synonym(s):
single-stranded template DNA
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About This Item
grade
for molecular biology
Quality Level
Assay
9-11 mg/mL
form
solution
concentration
10.0 ± 1.0 mg/mL in H2O
shipped in
dry ice
storage temp.
−20°C
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General description
Deoxyribonucleic acid, single stranded from salmon testes is a ready-to-use solution of high quality, sonicated, single-stranded template DNA isolated from the testes of salmon.
Application
Deoxyribonucleic acid, single stranded from salmon testes has been used for/as:
- in situ hybridization histochemistry of rat brain, human kidney sections and renal biopsy tissues
- preparing standard curve of DNA
- prehybridization solution in southern and northern hybridizations
- dilution of the cDNA
- fluorescent in-situ hybridization (FISH)
- ChIP analysis
- a standard to analyse the degraded nucleosides of genomic DNA by high performance liquid chromatography
- a component of hybridization solution in FISH
- a control in DNA-DNA hybridization experiment between various bacterial species
- to precipitate probes in fluorescence in situ hybridization (FISH)
Many factors contribute to the signal-to-noise ratio in nucleic acid hybridizations. These factors include the presence of solvent (formamide), hybridization temperature, length of hybridization, volume of hybridization solution, degree and method of agitation, use of blocking reagents, concentration and specific activity of the probe, use of molecular agents to increase the rate of nucleic acid reassociation, and the degree of stringency used during the washing of the membrane.
In order to decrease any non-specific hybridization of the probe to a substrate, blocking agents must be used. Generally, a combination of blocking reagent, detergent, and denatured, fragmented DNA is used to accomplish this. Sigma offers sonicated, denatured DNA from a variety of species for use as a blocking agent in Northern and Southern blotting and other nucleic acid hybridization techniques.
In order to decrease any non-specific hybridization of the probe to a substrate, blocking agents must be used. Generally, a combination of blocking reagent, detergent, and denatured, fragmented DNA is used to accomplish this. Sigma offers sonicated, denatured DNA from a variety of species for use as a blocking agent in Northern and Southern blotting and other nucleic acid hybridization techniques.
Suitable for use as a blocking agent in Southern hybridizations.
Preparation Note
This DNA is phenol-chloroform extracted, ethanol precipitated, and sonicated to produce single-stranded fragments which comigrate with the 587 and 831 base pair marker fragments.
Other Notes
DNA in solution will reanneal on standing at room temperature so it is recommended to boil the solution for 10 minutes and then cool on ice for at least 5 minutes prior to use.
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Product No.
Description
Pricing
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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Rune Thomsen et al.
PloS one, 8(8), e72110-e72110 (2013-08-31)
The intermediate filament network of astrocytes includes Glial fibrillary acidic protein (Gfap) as a major component. Gfap mRNA is alternatively spliced resulting in generation of different protein isoforms where Gfapα is the most predominant isoform. The Gfapδ isoform is expressed
L Kölby et al.
British journal of cancer, 89(7), 1383-1388 (2003-10-02)
The radio-iodinated noradrenaline analogue meta-iodobenzylguanidine (MIBG) can be used for scintigraphy and radiation therapy of neuroendocrine (NE). The aim of the present study was to study the importance of vesicular monoamine transporters (VMATs) for the uptake of (123)I-MIBG in NE
Sydney Tang et al.
The Journal of clinical investigation, 111(4), 515-527 (2003-02-18)
Renal tubulointerstitial injury is characterized by inflammatory cell infiltrate; however, the stimuli for leukocyte recruitment are not fully understood. IL-8 is a potent chemokine produced by proximal tubular epithelial cells (PTECs). Whether nephrotic proteins stimulate tubular IL-8 expression remains unknown.
I Shibuya et al.
The Journal of physiology, 514 ( Pt 2), 351-367 (1998-12-16)
1. The expression, distribution and function of P2X purinoceptors in the supraoptic nucleus (SON) were investigated by reverse transcription-polymerase chain reaction (RT-PCR), in situ hybridization, and Ca2+-imaging and whole-cell patch-clamp techniques, respectively. 2. RT-PCR analysis of all seven known P2X
D Suzuki et al.
Diabetes, 44(10), 1233-1238 (1995-10-01)
Increased mesangial expansion is one of the most characteristic histological changes in diabetic nephropathy (DN). Although the pathogenesis of DN remains unclear, recent studies associate interleukin (IL) 6 with mesangial proliferative glomerulonephritis. To elucidate the expression and localization of IL-6
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Available Fluorescent in situ hybridization (FISH) procedures, reagents and equipment.
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