N6019
Neutralizing Buffer
for Northern and Southern blotting, powder
Synonym(s):
Neutralizing Buffer for Blotting Techniques
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About This Item
UNSPSC Code:
12161700
NACRES:
NA.25
grade
for molecular biology
Quality Level
form
powder
technique(s)
Southern blotting: suitable
foreign activity
DNase, RNase, none detected
storage temp.
room temp
Related Categories
General description
Sigma′s neutralization buffer is suitable for Southern blotting procedures to neutralize the pH of the gel following depurination and denaturation treatment or before transferring it to membranes.
Application
Suitable for neutralizing the gel before transfer to membranes during Southern procedures.
Components
The Neutralization Buffer is composed of 1M Tris-HCl and 1.5M NaCl.
related product
Product No.
Description
Pricing
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Find documentation for the products that you have recently purchased in the Document Library.
A southern blot assay for detection of hepatitis B virus covalently closed circular DNA from cell cultures
Cai D, et al.
Methods in Molecular Biology, 151-161 (2013)
Ausubel, F.M., et al.
Short Protocols in Molecular Biology (1995)
Sambrook, J., and Russell, D. W.
Molecular Cloning: A Laboratory Manual, 6-6 (2001)
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 9-9 (1989)
Protocols
Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.
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