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N1531

Sigma-Aldrich

Denaturation Solution

for Northern and Southern blotting

Synonym(s):

Denaturation Solution For Blotting Technique, Denaturation Solution for Neutral Southern Transfer

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Select a Size

5 G
CN¥508.18
25 G
CN¥2,645.56
50 G
CN¥4,603.87

CN¥508.18

List PriceCN¥846.97Save 40%

Available to ship onApril 17, 2025Details

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5 G
CN¥508.18
25 G
CN¥2,645.56
50 G
CN¥4,603.87

About This Item

UNSPSC Code:
12161700
NACRES:
NA.25

CN¥508.18

List PriceCN¥846.97Save 40%

Available to ship onApril 17, 2025Details

grade

Molecular Biology

Quality Level

200

form

solution

storage temp.

room temp

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Show Differences

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154903PHR1372439193
Methanol UHPLC, suitable for mass spectrometry (MS)

900688

Methanol

Methanol ACS spectrophotometric grade, ≥99.9%

154903

Methanol

Methanol Pharmaceutical Secondary Standard; Certified Reference Material

PHR1372

Methanol

Methanol suitable for HPLC, gradient grade, suitable as ACS-grade LC reagent, ≥99.9%

439193

Methanol

assay

≥99.92%

assay

≥99.9%

assay

-

assay

≥99.9%

application(s)

food and beverages

application(s)

food and beverages

application(s)

pharmaceutical (small molecule)

application(s)

food and beverages

technique(s)

UHPLC: suitable, mass spectrometry (MS): suitable

technique(s)

UV/Vis spectroscopy: suitable

technique(s)

HPLC: suitable, gas chromatography (GC): suitable

technique(s)

HPLC: suitable

form

liquid

form

liquid

form

-

form

liquid

vapor density

1.11 (vs air)

vapor density

1.11 (vs air)

vapor density

1.11 (vs air)

vapor density

1.11 (vs air)

General description

Sigma′s denaturing solution is a strong base that denatures double-stranded DNA so that it can be efficiently transferred to nylon membrane and subsequently hybridized to labeled probe. When used with a depurination solution, treatment results in the hydrolysis of the phosphodiester backbone at the sites of depurination, allowing for more efficient transfer of large DNA in neutral Southern transfers.

Application

Denaturation Solution is suitable for denaturing dsDNA before the transfer to membranes during Northern and Southern blotting procedures.

Components

The denaturing solutions is composed of 0.5N NaOH, and 1.5M NaCl.

Other Notes

0.5 N NaOH, 1.5 M NaCl

related product

Product No.
Description
Pricing

Pictograms

Corrosion
GHS05

Signal Word

Danger

Hazard Statements

H290,H314

Hazard Classifications

Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1B

Storage Class Code

8B - Non-combustible corrosive hazardous materials

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

  • Specification Sheet

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    Certificates of Analysis (COA)

    Lot/Batch Number
    0000438535
    0000438535
    0000340598
    0000340598
    0000301763

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    José Manuel Pérez-Pérez et al.
    Plant physiology, 134(1), 101-117 (2004-01-20)
    The dwarf ucu (ultracurvata) mutants of Arabidopsis display vegetative leaves that are spirally rolled downwards and show reduced expansion along the longitudinal axis. We have previously determined that the UCU1 gene encodes a SHAGGY/GSK3-like kinase that participates in the signaling
    Sambrook, J., et al.
    Molecular Cloning: A Laboratory Manual, 9-9 (1989)
    Ausubel, F.M., et al.
    Short Protocols in Molecular Biology (1995)

    Protocols

    Northern & Southern Blot Protocols

    Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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