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Key Documents

S6639

Sigma-Aldrich

SSC Buffer 20× Concentrate

for Northern and Southern blotting, solution

Synonym(s):

20X SSC Buffer, SSC hybridization buffer, Saline-sodium citrate buffer

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About This Item

UNSPSC Code:
41105319
NACRES:
NA.25

¥16,094.23

Available to ship on2025年4月27日Details

Request a Bulk Order

grade

Molecular Biology

Quality Level

200

description

Saline-Sodium Citrate buffer, made with ultrapure water

sterility

0.2 μm filtered

form

solution

application(s)

diagnostic assay manufacturing

foreign activity

DNAse, none detected
Endonuclease, none detected
Exonuclease, none detected
NICKase, none detected
RNAse, none detected

storage temp.

room temp

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separation technique

ion exclusion

separation technique

ligand exchange

separation technique

ion exclusion

separation technique

ion exclusion

particle size

9 μm

particle size

9 μm

particle size

9 μm

particle size

9 μm

matrix

sulfonated polystyrene/divinylbenzene, spherical particle platform, polymeric

matrix

sulfonated polystyrene/divinylbenzene, spherical particle platform, polymeric

matrix

sulfonated polystyrene/divinylbenzene, spherical particle platform, polymeric

matrix

sulfonated polystyrene/divinylbenzene, spherical, polymeric

product line

SUPELCOGEL™

product line

SUPELCOGEL™

product line

SUPELCOGEL™

product line

SUPELCOGEL™

agency

suitable for USP L17

agency

suitable for USP L19

agency

suitable for USP L17

agency

suitable for USP L17

technique(s)

HPLC: suitable

technique(s)

HPLC: suitable

technique(s)

HPLC: suitable

technique(s)

HPLC: suitable

General description

SSC (Saline Sodium Citrate) Buffer 20x liquid is a standard reagent in Southern and Northern hybridization procedures. It is used to control stringency of wash buffer for the washing steps after hybridization.

Application

SSC Buffer 20× Concentrate has been used
  • as a hybridization buffer on in situ hybridization human-specific repetitive Alu sequence[1]
  • as a washing buffer for membrane for Southern hybridization, dot blot of mice genomic DNA[2] and northern blotting of microRNA[3]
  • as an incubation buffer in fluorescence in situ hybridization (FISH) of L1 retrotransposon[4]

Features and Benefits

  • Convenient ready-to-use liquid
  • Multi-purpose use in hybridizations

Preparation Note

SSC Buffer 20x contains 3M NaCl in 0.3M sodium citrate (pH 7.0).

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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    Certificates of Analysis (COA)

    Lot/Batch Number
    0000339589
    0000339589
    0000401942
    0000379454
    0000356424

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    Chronic hypoxia facilitates Alzheimer's disease through demethylation of gamma-secretase by downregulating DNA methyltransferase 3b
    Liu H, et al.
    Alzheimers Dement., 12(2), 130-143 (2016)
    L1 retrotransposon expression in circulating tumor cells
    Papasotiriou I, et al.
    Testing, 12(2), e0171466-e0171466 (2017)
    Chang-Gong Liu et al.
    Methods (San Diego, Calif.), 44(1), 22-30 (2007-12-26)
    After 12 years from its first application, microarray technology has become the reference technique to monitor gene expression of thousands of genes in the same experiment. In the past few years an increasing amount of evidence showed the importance of
    M Böttger et al.
    Nucleic acids research, 9(20), 5253-5268 (1981-10-24)
    Complexes of histones H1 with superhelical SV40 DNA obtained by direct mixing were studied in 0.1 SSC buffer corresponding to 0.02 M Na+. Depending on the molar input ratio H1/DNA three classes of sedimenting species were observed: (1) a component
    Michaela Asp et al.
    Scientific reports, 7(1), 12941-12941 (2017-10-13)
    Heart failure is a major health problem linked to poor quality of life and high mortality rates. Hence, novel biomarkers, such as fetal marker genes with low expression levels, could potentially differentiate disease states in order to improve therapy. In
    View All Related Papers

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    Protocols

    Northern & Southern Blot Protocols

    Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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