跳转至内容

CN

Quantification of 3-MCPD and its mercapturic metabolite in human urine: validation of an LC-MS-MS method and its application in the general population.

Quantification of 3-MCPD and its mercapturic metabolite in human urine: validation of an LC-MS-MS method and its application in the general population.

Analytical and bioanalytical chemistry (2015-04-12)

Roberta Andreoli, Martina Cirlini, Antonio Mutti

摘要

A new method for the simultaneous quantitative determination in human urine of 3-monochloropropane-1,2-diol (3-MCPD), a toxic food contaminant, and its metabolite, 2,3-dihydroxypropyl mercapturic acid (DHPMA), was developed and validated. After urine dilution, the analytes were separated on an Atlantis®dC18 column and quantified by liquid chromatography-tandem mass spectrometry using isotopically labelled internal standards. The limits of quantification (S/N = 10) were 1.90 and 2.21 μg/L for 3-MCPD and DHPMA, respectively. Intra- and inter-day precision were lower than 6 % for each compound. Matrix effects were evaluated. Due to the high sensitivity and good accuracy of the method, 3-MCPD and DHPMA were found in 67 and 100 % of urine samples of healthy subjects, respectively.

材料

货号

品牌

产品描述

Sigma-Aldrich

乙腈, anhydrous, 99.8%

Sigma-Aldrich

纯乙醇, 200 proof, anhydrous, ≥99.5%

Sigma-Aldrich

硫酸, 99.999%

Sigma-Aldrich

三乙基胺, ≥99.5%

Sigma-Aldrich

甲醇, anhydrous, 99.8%

Sigma-Aldrich

三乙基胺, ≥99%

Sigma-Aldrich

L-半胱氨酸盐酸盐, anhydrous, ≥98% (TLC)

Sigma-Aldrich

乙酸酐, ReagentPlus^®, ≥99%

Sigma-Aldrich

纯乙醇, 190 proof, ACS spectrophotometric grade, 95.0%

Sigma-Aldrich

三乙基胺, BioUltra, ≥99.5% (GC)

Sigma-Aldrich

三乙基胺, puriss. p.a., ≥99.5% (GC)

Sigma-Aldrich

乙酸, natural, ≥99.5%, FG

Sigma-Aldrich

正丁醇, for molecular biology, ≥99%

Sigma-Aldrich

乙酸, ≥99.5%, FCC, FG

Sigma-Aldrich

乙酸酐, ACS reagent, ≥98.0%

Sigma-Aldrich

正丁醇, anhydrous, 99.8%

Sigma-Aldrich

乙酸, for luminescence, BioUltra, ≥99.5% (GC)

Sigma-Aldrich

(±)-3-氯-1,2-丙二醇, 98%

Sigma-Aldrich

L-半胱氨酸盐酸盐, anhydrous, from non-animal source, BioReagent, suitable for cell culture, ≥98.0%

Sigma-Aldrich

L-半胱氨酸盐酸盐, anhydrous, ≥99.0% (RT)

Sigma-Aldrich

丙酮, natural, ≥97%

Sigma-Aldrich

丙酮, ≥99%, meets FCC analytical specifications

Sigma-Aldrich

丁醇, natural, ≥99.5%, FCC, FG

Sigma-Aldrich

三乙基胺, for amino acid analysis, ≥99.5% (GC)

Sigma-Aldrich

纯乙醇, 190 proof, meets USP testing specifications

Sigma-Aldrich

乙腈, electronic grade, 99.999% trace metals basis

Supelco

10% (v/v) 乙醇标准品, 10 % (v/v) in H₂O, analytical standard

Sigma-Aldrich

三乙基胺, for protein sequence analysis, ampule, ≥99.5% (GC)

Sigma-Aldrich

甲醇, NMR reference standard

Sigma-Aldrich

甲醇溶液, NMR reference standard, 4% in methanol-d₄ (99.8 atom % D), NMR tube size 3 mm × 8 in.