HPLC Analysis of Cytochrome c Species Variants on Discovery^® BIO Wide Pore C18

技术

HPLC: suitable

试验参数

column: Discovery BIO Wide Pore C18, 15 cm × 4.6 mm I.D., 5 μm particles (568222-U)
mobile phase: [A] (0.1% TFA in water):(0.1% TFA in acetonitrile); (70:30), [B] (0.1% TFA in water):(0.1% TFA in acetonitrile), 64:36
gradient: 0-100% B in 30 min
flow rate: 1 mL/min
column temp.: ambient
detector: UV, 220 nm
injection: 12 μL each at 0.8 mg/mL in 0.1%TFA

适用性

application for HPLC

应用

life science and biopharma