product name
Discovery ® HS F5 HPLC 色谱柱, 5 μm particle size, L × I.D. 25 cm × 4.6 mm
物料
stainless steel column
质量水平
Agency
suitable for USP L43
产品线
Discovery®
特点
endcapped
制造商/商品名称
Discovery®
包装
1 ea of
标记范围
12% Carbon loading
参数
≤70 °C temp. range
400 bar pressure (5801 psi)
技术
HPLC: suitable
LC/MS: suitable
长度 × 内径
25 cm × 4.6 mm
表面积
300 m2/g
表面覆盖度
4 μmol/m2
杂质
<10 ppm metals
基质
silica gel, high purity, spherical particle platform
fully porous particle
基质活性基团
PFP (pentafluorophenyl) phase
粒径
5 μm
孔径
120 Å
operating pH range
2-8
应用
food and beverages
分离技术
reversed phase
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相关类别
一般描述
将 C18 柱方法转换至 Discovery® HS F5 柱的指导原则：
通常，碱性化合物在 HS F5 色谱柱上的保留时间比在 C18 柱上更长。在 C18 柱上流动相中的有机相含量提高 5% 至 10% 通常可获得与 HS F5 柱类似的保留值。使用其他化合物所得的结果差异则很大。然而 log Po/w 值小于 2.5 的化合物在 HS F5 柱上的保留时间通常要长于 C18 柱。保留时间的差异程度与化合物的 logP o/w 值密切相关。
通常，碱性化合物在 HS F5 色谱柱上的保留时间比在 C18 柱上更长。在 C18 柱上流动相中的有机相含量提高 5% 至 10% 通常可获得与 HS F5 柱类似的保留值。使用其他化合物所得的结果差异则很大。然而 log Po/w 值小于 2.5 的化合物在 HS F5 柱上的保留时间通常要长于 C18 柱。保留时间的差异程度与化合物的 logP o/w 值密切相关。
Discovery® HS F5 键合相可提供与 C18 色谱柱截然不同的反相分离。由于 F5 色谱柱上各种化合物通常会在与 C18 相同的时间范围内洗脱出来，使得大部分的 C18 方法可轻松转换到 F5 上。
应用
- Determination of 6-hydroxy-1-methyl-1,2,3,4-tetrahydro-beta-carboline, 5-hydroxytryptamine and 5-hydroxyindole acetic acid in the neonatal rat brains using high performance liquid chromatography-electrochemical detection.: This study highlights the use of the Discovery® HS F5 HPLC Column for the sensitive detection of neuroactive compounds in neonatal rat brain samples, advancing our understanding of neurological development and neurotransmitter dynamics (Mao et al., 2009).
- Advantages of pentafluorophenylpropyl stationary phase over conventional C18 stationary phase--application to analysis of triamcinolone acetonide.: This research showcases the superior performance of the Discovery® HS F5 HPLC Column in analyzing corticosteroid formulations, providing insights that could lead to improved analytical methodologies in pharmaceutical analysis (Havlikova et al., 2008).
- Determination of ephedrine alkaloids in Ephedra natural products using HPLC on a pentafluorophenylpropyl stationary phase.: Utilizes the Discovery® HS F5 HPLC Column for the precise and efficient separation of ephedrine alkaloids, contributing to safer and more effective use of Ephedra in herbal supplements (Pellati and Benvenuti, 2008).
- Fast high-performance liquid chromatography analysis of phenethylamine alkaloids in Citrus natural products on a pentafluorophenylpropyl stationary phase.: Demonstrates the efficiency of the Discovery® HS F5 HPLC Column in the rapid analysis of phenethylamine alkaloids, enhancing the quality control of Citrus-based dietary supplements (Pellati and Benvenuti, 2007).
- High-performance liquid chromatographic method with UV photodiode-array, fluorescence and mass spectrometric detection for simultaneous determination of galantamine and its phase I metabolites in biological samples.: This study uses the Discovery® HS F5 HPLC Column to simultaneously quantify galantamine and its metabolites, aiding in the pharmacokinetic profiling of this Alzheimer′s disease medication (Malakova et al., 2007).
特点和优势
- 独特的选择性
- 保留能力与 C18 柱类似（有时需要洗脱能力更强的流动相）
- 峰形极佳
- 稳定性好、低流失，适用于 LC-MS 分析
- 粒径范围从 3μm 到 10μm 可供选择
法律信息
Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany
Jennie Wang et al.
Journal of pharmaceutical and biomedical analysis, 124, 267-273 (2016-03-13)
An HPLC method for the assay of a DNA topoisomerase inhibitor, LMP776 (NSC 725776), has been developed and validated. The stress testing of LMP776 was carried out in accordance with International Conference on Harmonization (ICH) guidelines Q1A (R2) under acidic
Umberto M Battisti et al.
Journal of chromatography. A, 1443, 152-161 (2016-03-30)
A "heart-cut" two-dimensional achiral-chiral liquid chromatography triple-quadrupole mass spectrometry method (LC-LC-MS/MS) was developed and coupled to in vivo cerebral microdialysis to evaluate the brain response to the chiral compound (±)-7-chloro-5-(3-furanyl)-3-methyl-3,4-dihydro-2H-1,2,4-benzothiadiazine-1,1-dioxide ((±)-1), a potent positive allosteric modulator (PAM) of AMPA receptor.
Lucie Havlíková et al.
Talanta, 76(3), 597-601 (2008-07-01)
A pentafluorophenylpropyl (PFPP) stationary phase was for the first time tested for the simultaneous determination of triamcinolone acetonide, its degradation product triamcinolone and two preservatives, methylparaben, and propylparaben. A new simple isocratic reversed phase HPLC method with UV detection, using
Jana Maláková et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 853(1-2), 265-274 (2007-04-10)
Galantamine, an alkaloid isolated from the bulbs and flowers of Caucasian snowdrop (Galanthus woronowii, Amaryllidaceae) and related species, is employed in human medicine for the treatment of various neuromuscular and neurodegenerative diseases. After the administration, the products of oxidative biotransformation
Ilia Brondz et al.
Journal of pharmaceutical and biomedical analysis, 43(3), 937-944 (2006-11-03)
The drug primaquine diphosphate is used for causative treatment of malaria. Using HPLC-MS and GC-MS, this research group was previously able to show that the main contaminant of primaquine is the positional isomer quinocide [I. Brondz, D. Mantzilas, U. Klein
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