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Merck
CN

567507-U

Discovery® HS F5 (3 µm) HPLC Columns

L × I.D. 15 cm × 4.6 mm, HPLC Column

别名:

Discovery五氟苯基PFP HPLC色谱柱

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UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52

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产品名称

Discovery ® HS F5 HPLC 色谱柱, 3 μm particle size, L × I.D. 15 cm × 4.6 mm

material

stainless steel column

agency

suitable for USP L43

product line

Discovery®

feature

endcapped

manufacturer/tradename

Discovery®

packaging

1 ea of

extent of labeling

12% Carbon loading

parameter

≤70 °C temp. range
400 bar pressure (5801 psi)

technique(s)

HPLC: suitable
LC/MS: suitable

L × I.D.

15 cm × 4.6 mm

surface area

300 m2/g

surface coverage

4 μmol/m2

impurities

<10 ppm metals

matrix

silica gel, high purity, spherical particle platform
fully porous particle

matrix active group

PFP (pentafluorophenyl) phase

particle size

3 μm

pore size

120 Å

operating pH range

2-8

application(s)

food and beverages

separation technique

reversed phase

Quality Level

100

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相关类别

Application

  • Nature of the main contaminant in the drug primaquine diphosphate: SFC and SFC-MS methods of analysis.：采用Discovery® HS F5 HPLC色谱柱鉴定和定量二磷酸伯氨喹中的主要污染物，对于确保这种药物的品质和安全性有重要意义(Brondz et al., 2007)。
  • HPLC assisted chemobiological standardization of alpha-glucosidase-I enzyme inhibitory constituents from Piper longum Linn-An Indian medicinal plant.：使用Discovery® HS F5 HPLC色谱柱进行印度药用植物的生物相关化学分析，助力草药标准化(Pullela et al., 2006)。
  • Disposition study of a new potential antineoplastic agent dimefluron in rats using high-performance liquid chromatography with ultraviolet and mass spectrometric detection.：利用Discovery® HS F5 HPLC色谱柱研究新型抗癌药物的药动学和代谢，助力开发有效的癌症药物(Císar et al., 2005)。
  • A simplified analytical method for a phenotyping cocktail of major CYP450 biotransformation routes.：采用Discovery® HS F5 HPLC色谱柱简化分型混合物(phenotyping cocktail)分析，改进药物代谢评估和个性化医疗方法(Jerdi et al., 2004)。

Features and Benefits

  • 独特的选择性
  • 保留能力与 C18 柱类似（有时需要洗脱能力更强的流动相）
  • 峰形极佳
  • 稳定性好、低流失，适用于 LC-MS 分析
  • 粒径范围从 3μm 到 10μm 可供选择

General description

将 C18 柱方法转换至 Discovery® HS F5 柱的指导原则：
通常，碱性化合物在 HS F5 色谱柱上的保留时间比在 C18 柱上更长。在 C18 柱上流动相中的有机相含量提高 5% 至 10% 通常可获得与 HS F5 柱类似的保留值。使用其他化合物所得的结果差异则很大。然而 log Po/w 值小于 2.5 的化合物在 HS F5 柱上的保留时间通常要长于 C18 柱。保留时间的差异程度与化合物的 logP o/w 值密切相关。
Discovery® HS F5 键合相可提供与 C18 色谱柱截然不同的反相分离。由于 F5 色谱柱上各种化合物通常会在与 C18 相同的时间范围内洗脱出来，使得大部分的 C18 方法可轻松转换到 F5 上。

Other Notes

探索最适于 HPLC 或 LC-MS 分析的 LiChropur 试剂

Legal Information

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
299023-06
299023-05
299023-04
299023-03
299023-02

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Hiroyuki Kataoka et al.
Analytical and bioanalytical chemistry, 405(1), 331-340 (2012-10-16)
We have developed a simple and sensitive method for the simultaneous determination of testosterone (TES), cortisol (CRT), and dehydroepiandrosterone (DHEA) in saliva by automated online in-tube solid-phase microextraction (SPME) coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS) using a Discovery HS
Milan Nobilis et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 879(32), 3845-3852 (2011-11-22)
New bioanalytical SPE-HPLC-PDA-FL method for the determination of the neuroleptic drug tiapride and its N-desethyl metabolite was developed, validated and applied to xenobiochemical and pharmacokinetic studies in humans and animals. The sample preparation process involved solid-phase extraction of diluted plasma
Federica Pellati et al.
Journal of chromatography. A, 1165(1-2), 58-66 (2007-08-07)
In this study, the chromatographic performance of a pentafluorophenylpropyl (PFPP) stationary phase was evaluated for the rapid separation of phenethylamine alkaloids (i.e. (+/-)-octopamine, (+/-)-synephrine, tyramine, N-methyltyramine and hordenine) in Citrus aurantium plant material (fruits and peel), various Citrus species, extracts
Federica Pellati et al.
Journal of pharmaceutical and biomedical analysis, 48(2), 254-263 (2007-12-14)
In this study a pentafluorophenylpropyl (PFPP) stationary phase was applied to the fast and reliable qualitative and quantitative analysis of ephedrine alkaloids in Ephedra plant material and derivatives. A Discovery HS F5 column (150mmx4.6mm i.d., 5microm) was used, with an
Ilia Brondz et al.
Journal of pharmaceutical and biomedical analysis, 43(3), 937-944 (2006-11-03)
The drug primaquine diphosphate is used for causative treatment of malaria. Using HPLC-MS and GC-MS, this research group was previously able to show that the main contaminant of primaquine is the positional isomer quinocide [I. Brondz, D. Mantzilas, U. Klein
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