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50632-U

Supelco

Ascentis® Express 90 Å F5 (5 μm) HPLC Columns

L × I.D. 25 cm × 4.6 mm, HPLC Column

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别名:
Core-shell (SPP) Fused Core PFP HPLC column
UNSPSC代码:
41115700
eCl@ss:
32110501
NACRES:
SB.52

product name

Ascentis® Express F5, 5 μm HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4.6 mm

物料

stainless steel column

质量水平

100

Agency

suitable for USP L43

产品线

Ascentis®

特点

endcapped

制造商/商品名称

Ascentis®

包装

1 ea of

参数

60 °C temp. range
600 bar max. pressure (9000 psi)

技术

HPLC: suitable
LC/MS: suitable

长度 × 内径

25 cm × 4.6 mm

表面积

90 m2/g

杂质

<5 ppm metals

基质

Fused-Core particle platform
superficially porous particle

基质活性基团

PFP (pentafluorophenyl) phase

粒径

5 μm

孔径

90 Å pore size

工作pH值

2-8

应用

food and beverages

分离技术

reversed phase

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相关类别

一般描述

Ascentis® Express 5 μm F5 is a high-speed, high-performance liquid chromatography column based on the highly efficient Fused-Core® particle design. The bonded, endcapped, dimethylpentafluorophenyl-propylsilane stationary phase of Ascentis Express 5 μm F5 provides a stable, reversed-phase packing with electron-deficient phenyl rings due to the presence of electronegative fluorines. In addition to forming π-π and mildly steric interactions, F5 phases also retain compounds by polar interactions. As a result of having both polar and non-polar character, F5 phases can show dual-mode retention behavior, sometimes producing a “U-shaped” retention as a function of acetonitrile content of the mobile phase, with retention increasing at both low and high concentrations of ACN (reversed-phase and HILIC retention modes). Ascentis Express 5 μm F5 can be used for basic, acidic, or neutral compounds with alternate selectivity from C18.

应用


  • Liquid chromatographic determination of lumacaftor in the presence of ivacaftor and identification of five novel degradation products using high-performance liquid chromatography ion trap time-of-flight mass spectrometry.: This study employs an Ascentis® Express F5, 5 µm HPLC column for the precise determination of lumacaftor amidst ivacaftor and identifies novel degradation products, showcasing the column′s efficacy in complex mixture analysis (Özcan et al., 2023).

  • Stability-indicating LC-MS/MS and LC-DAD methods for robust determination of tasimelteon and high resolution mass spectrometric identification of a novel degradation product.: Utilizing the Ascentis® Express F5, 5 µm HPLC column, this research developed stability-indicating methods for tasimelteon, highlighting the column′s robustness in pharmaceutical analysis (Özcan et al., 2020).

  • Recent trends in ultra-fast HPLC: new generation superficially porous silica columns.: This review explores the advancements in HPLC columns, including the Ascentis® Express F5, emphasizing its role in achieving ultra-fast separations in analytical chemistry (Ali et al., 2012).

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法律信息

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Fused-Core is a registered trademark of Advanced Materials Technology, Inc.

WGK

nwg

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

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Iltaf Shah et al.
The Journal of steroid biochemistry and molecular biology, 180, 118-128 (2018-02-13)
Research shows that immunoassay techniques are not the best choice for the estimation of vitamin D in human blood samples. The main reasons are that some immunoassays are not able to distinguish between 25-OHD3 and 25-OHD2 vitamin D metabolites. Furthermore
Maria A Khalikova et al.
Talanta, 130, 433-441 (2014-08-28)
The presented work describes the development of a simple, fast and effective on-line SPE-UHPLC-UV/vis method using fused core particle columns for extraction, separation and quantitative analysis of the nine illegal dyes, most frequently found in chilli-containing spices. The red dyes
Alex D Batista et al.
Talanta, 133, 142-149 (2014-12-02)
On-line sample pretreatment (clean-up and analyte preconcentration) is for the first time coupled to sequential injection chromatography. The approach combines anion-exchange solid-phase extraction and the highly effective pentafluorophenylpropyl (F5) fused-core particle column for separation of eight sulfonamide antibiotics with similar
Jan Soukup et al.
Journal of chromatography. A, 1374, 102-111 (2014-12-30)
Excess adsorption of water from aqueous acetonitrile mobile phases was investigated on 16 stationary phases using the frontal analysis method and coulometric Karl-Fischer titration. The stationary phases include silica gel and silica-bonded phases with different polarities, octadecyl and cholesterol, phenyl
Imran Ali et al.
Journal of separation science, 35(23), 3235-3249 (2012-11-28)
New generation columns, i.e. packed with superficially porous silica particles are available as trade names with following manufacturers: Halo, Ascentis Express, Proshell 120, Kinetex, Accucore, Sunshell, and Nucleoshell. These provide ultra-fast HPLC separations for a variety of compounds with moderate
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