Bst Max DNA Polymerase

Loop-mediated isothermal amplification (LAMP) has emerged as a key alternative to polymerase chain reaction (PCR)-based techniques in the amplification of nucleic acids. While conventional PCR employs a thermal cycler for repetitive cycling temperatures when amplifying, isothermal amplification techniques such as LAMP occur at a single and fixed temperature allowing the use of simple, portable, and more robust instruments for fast and exponential amplification.
The Bst Max DNA polymerase is an in-silicon designed homolog of Bst DNA Polymerase (large fragment) suitable for DNA amplification at elevated temperatures with an optimum of 65°C. Bst Max DNA polymerase is a salt-tolerant recombinant polymerase (optimal salt concentration 50-350 mM) with more than two-fold enhanced strand-displacement activity and processivity when compared to Bst polymerase. The Bst enzyme is active from 25 to 65°C. It lacks 5’-3’- and 3’-5’-exonuclease activity.

Bst Max DNA Polymerase is

• Ideal for isothermal applications such as LAMP and RT-LAMP for its superior amplification performance and robustness, especially at point-of-care diagnostics
• Compatible with a wide range of buffer formulations and isothermal applications such as Ramification amplification (RAM), NEMA (Nicking enyzme-mediated amplification), HAD (Helicase-dependent amplification), MDA/SDA (Multiple/Strand displacement amplification), RCA (Rolling circle amplification), RPA (Recombinase Polymerase amplification)

One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 65°C.

In solution.

Order primers for LAMP using our online configurator. For assistance with primer design prior to purchase, please use the online design tool from our partner.