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一般描述
应用
- PCR扩增昆虫、 真菌、病毒、人类来源分离的基因组DNA
- 总RNA逆转录为cDNA
- 作为聚合酶链式反应(PCR)DNA扩增混合物组分
- 常规和长片段PCR
- 手动和自动DNA测序
- cDNA合成和标记反应
特点和优势
- 每种dNTP的纯度：最低99%
- 方便配制；每50 μL PCR使用1 μL
- 等摩尔量的各种dNTP可减少移液步骤
- 最大限度地降低PCR污染风险
- UltraPure超纯dNTP可帮助最大限度地提高关键PCR反应的一致性和得率
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
What are the components to Product D7295, Deoxynucleotide Mix?
The D7295 contains 10mM of each dATP, dCTP, dGTP and TTP in molecular biology grade water. This is for a total concentration of 40 mM.
Does Product No. D7295, Deoxynucleotide Mix contain dTTP or TTP?
The product contains Thymidine 5´-triphosphate sodium salt. Thymidine has a deoxy sugar, so it is a deoxythymidine.
How much Product D7295, Deoxynucleotide Mix, is needed for a PCR reaction?
One μl is sufficient for a standard 50 μl PCR reaction.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
实验方案
Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures. AccuTaq LA.
Method for amplification of DNA from damaged DNA sources. Particularly useful for DNA extracted from old samples.
Protocol using antibody mediated hot start polymerase with a red dye for easy gel loading. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.
MTP™ Taq DNA Polymerase is a recombinant thermostable enzyme from Thermus aquaticus expressed in E. coli and purified using a proprietary process to minimize levels of contaminating DNA.
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