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主要文件

安全信息

P2192

Sigma-Aldrich

10X PCR缓冲液

Optimized for routine PCR with MgCl2 included

别名:

PCR反应缓冲液, PCR缓冲溶液

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选择尺寸

100 MG
CN¥785.64

CN¥785.64


国内现货,预计发货时间April 24, 2025详情



选择尺寸

变更视图
100 MG
CN¥785.64

About This Item

UNSPSC代码:
41106306
NACRES:
NA.52

CN¥785.64


国内现货,预计发货时间April 24, 2025详情


质量水平

表单

liquid

包装

vial of 1.5 mL

颜色

colorless

应用

agriculture

异质活性

DNase, RNase, none detected

储存温度

−20°C

比较类似商品

查看完整比较结果

显示差异

1 of 4

此商品
344827551850309866
assay

≥99%

assay

≥98% (NMR)

assay

≥98% (HPLC)

assay

≥98% (HPLC)

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

Quality Level

100

Quality Level

300

Quality Level

100

Quality Level

100

storage temp.

10-30°C

storage temp.

−20°C

storage temp.

10-30°C

storage temp.

10-30°C

storage condition

OK to freeze, protect from light

storage condition

OK to freeze, protect from light

storage condition

OK to freeze, desiccated (hygroscopic), protect from light

storage condition

OK to freeze, desiccated (hygroscopic)

应用

10X PCR缓冲液已用作PCR混合物组分:
  • 用于病毒DNA扩增[1]
  • 扩增基因组DNA,研究注意缺陷多动障碍(ADHD)患者的亚甲基四氢叶酸还原酶(MTHFR)基因突变[2]
  • 扩增芽孢杆菌来源的16S-23S rRNA基因内转录间隔区(ITS)[3]

特点和优势

  • 独立缓冲液
  • 可与JumpStart Taq DNA聚合酶(D9307)、来自栖热水生菌的Taq DNA聚合酶 (D1806)和REDTaq® 基因组DNA聚合酶(D8312)兼容

制备说明

Composition of the 10× buffer: 100 mM Tris-HCl, pH 8.3 at 25°C; 500 mM KCl; 15 mM MgCl2; 0.01% gelatin

法律信息

JumpStart is a trademark of Sigma-Aldrich Co. LLC
REDTaq is a registered trademark of Merck KGaA, Darmstadt, Germany

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves

法规信息

新产品
  • 技术规格说明书

  • 历史批次信息供参考:

    分析证书(COA)

    Lot/Batch Number

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    访问文档库

    G J Breedveld et al.
    Neurology, 59(4), 579-584 (2002-08-28)
    Benign hereditary chorea (BHC) is an autosomal dominant disorder that can be distinguished from Huntington disease by its early onset, stable or only slightly progressive course, and absence of mental deterioration. The variation in clinical features is such that its
    Maike Stam et al.
    Genetics, 162(2), 917-930 (2002-10-26)
    Paramutation is an interaction between alleles that leads to a heritable change in the expression of one allele. In B'/B-I plants, B-I (high transcription) always changes to B' (low transcription). The new B' allele retains the low expression state in
    Ákos Boros et al.
    PloS one, 6(12), e29145-e29145 (2011-12-24)
    A novel positive-sense, single-stranded RNA (+ssRNA) virus (Halastavi árva RNA virus, HalV; JN000306) with di-cistronic genome organization was serendipitously identified in intestinal contents of freshwater carps (Cyprinus carpio) fished by line-fishing from fishpond "Lőrinte halastó" located in Veszprém County, Hungary.
    Genome subtyping of autochthonous Bacillus species isolated from Iru, a fermented Parkia biglobosa seed
    Adewumi G A, et al.
    Food Biotechnology, 28(3), 250-268 (2014)
    Phylogenetic Analysis of Baculovirus Isolates from Diseased Insects in Southern Vietnam
    Thao N T P, et al.
    Open Journal of Genetics, 2014 (2014)

    实验方案

    Protocol using antibody mediated hot start polymerase with a red dye for easy gel loading. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.

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