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Merck
CN

P2192

10X PCR缓冲液

Optimized for routine PCR with MgCl2 included

别名:

PCR反应缓冲液, PCR缓冲溶液

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关于此项目

NACRES:
NA.52
UNSPSC Code:
41106306
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Quality Level

form

liquid

packaging

vial of 1.5 mL

color

colorless

application(s)

agriculture

foreign activity

DNase, RNase, none detected

storage temp.

−20°C

Application

10X PCR缓冲液已用作PCR混合物组分:
  • 用于病毒DNA扩增
  • 扩增基因组DNA,研究注意缺陷多动障碍(ADHD)患者的亚甲基四氢叶酸还原酶(MTHFR)基因突变
  • 扩增芽孢杆菌来源的16S-23S rRNA基因内转录间隔区(ITS)

Features and Benefits

  • 独立缓冲液
  • 可与JumpStart Taq DNA聚合酶(D9307)、来自栖热水生菌的Taq DNA聚合酶 (D1806)和REDTaq® 基因组DNA聚合酶(D8312)兼容

Preparation Note

10×缓冲液的组成:100mM Tris-HCl(pH8.3,25℃);500mM KCl;15mM MgCl2;0.01%明胶

Legal Information

JumpStart is a trademark of Sigma-Aldrich Co. LLC
REDTaq is a registered trademark of Merck KGaA, Darmstadt, Germany


存储类别

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves

法规信息

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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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实验方案

Protocol using antibody mediated hot start polymerase with a red dye for easy gel loading. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.


Phylogenetic Analysis of Baculovirus Isolates from Diseased Insects in Southern Vietnam
Thao N T P, et al.
Open Journal of Genetics, 2014 (2014)
Ákos Boros et al.
PloS one, 6(12), e29145-e29145 (2011-12-24)
A novel positive-sense, single-stranded RNA (+ssRNA) virus (Halastavi árva RNA virus, HalV; JN000306) with di-cistronic genome organization was serendipitously identified in intestinal contents of freshwater carps (Cyprinus carpio) fished by line-fishing from fishpond "Lőrinte halastó" located in Veszprém County, Hungary.
Maike Stam et al.
Genetics, 162(2), 917-930 (2002-10-26)
Paramutation is an interaction between alleles that leads to a heritable change in the expression of one allele. In B'/B-I plants, B-I (high transcription) always changes to B' (low transcription). The new B' allele retains the low expression state in



全球贸易项目编号

货号GTIN
P2192-5ML04061835507122
P2192-25ML04061834362692
P2192-1VL04061834362685
P2192-5VL04061834362708