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主要文件

P8787

Sigma-Aldrich

邻苯二胺 二盐酸盐

chromogenic, tablet

别名:

1,2-Phenylenediamine tablet, OPD, OPD Tablet

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1 KIT
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¥5,282.92


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1 KIT
¥5,282.92

About This Item

线性分子式:
C6H4(NH2)2·2HCl
CAS号:
分子量:
181.06
Beilstein:
3912045
MDL编号:
UNSPSC代码:
12352204
PubChem化学物质编号:
NACRES:
NA.83

¥5,282.92


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新价格,新优惠!

产品名称

邻苯二胺 二盐酸盐, tablet, 4 mg substrate per tablet

质量水平

表单

tablet

mp

258 °C (dec.) (lit.)

储存温度

2-8°C

SMILES字符串

Cl[H].Cl[H].Nc1ccccc1N

InChI

1S/C6H8N2.2ClH/c7-5-3-1-2-4-6(5)8;;/h1-4H,7-8H2;2*1H

InChI key

RIIWUGSYXOBDMC-UHFFFAOYSA-N

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RAB0469RAB0473RAB0467
Gene Information

mouse ... Timp1(21857)

Gene Information

mouse ... Timp1(21857)

Gene Information

mouse ... Timp2(21858)

Gene Information

human ... TIMP1(7076)

species reactivity

mouse

species reactivity

mouse

species reactivity

mouse

species reactivity

human

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 3 pg/mL
standard curve range: 1.37-1000 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 3 pg/mL
standard curve range: 1.37-1000 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 80 pg/mL
standard curve range: 0.0823-60 ng/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 30 pg/mL
standard curve range: 24.69-18000 pg/mL

input

sample type cell culture supernatant(s)
sample type plasma
sample type serum

input

sample type cell lysate
sample type tissue lysate

input

sample type cell culture supernatant(s)
sample type plasma
sample type serum

input

sample type tissue lysate
sample type cell lysate

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

一般描述

邻苯二胺二盐酸盐(OPD)是一种显色底物,因为利用辣根过氧化物酶缀合物,因此可用于酶联免疫吸附试验(ELISA)程序。底物产生可溶的最终产物,其颜色为橙棕色,并且可以在450 m下通过分光光度计读取。可以用3N HCl或3M H2SO4终止OPD反应,并在492 nm处读取。

应用

在酶联免疫吸附试验(ELISA)中,邻苯二胺盐酸盐用作辣根过氧化物酶偶联二抗的底物。

重悬

将 1 片片剂溶于 pH 值为 5.0的0.05M磷酸盐 - 柠檬酸盐缓冲液中至所需浓度(通常使用0.4mg / ml的OPD浓度)。临用前,向 100 mL 底物缓冲液中加入 40μl 新鲜过氧化氢(浓度为 30%)。片剂单独包装在铝箔包装中。

警示用语:

Warning

危险分类

Aquatic Chronic 2 - Carc. 2 - Muta. 2 - Skin Sens. 1

储存分类代码

11 - Combustible Solids

WGK

WGK 3


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