所有图片(2)
About This Item
线性分子式:
C6H4(NH2)2·2HCl
CAS号:
分子量:
181.06
Beilstein:
3912045
MDL编号:
UNSPSC代码:
12352204
PubChem化学物质编号:
NACRES:
NA.83
一般描述
邻苯二胺二盐酸盐（OPD）是一种显色底物，因为利用辣根过氧化物酶缀合物，因此可用于酶联免疫吸附试验（ELISA）程序。底物产生可溶的最终产物，其颜色为橙棕色，并且可以在450 m下通过分光光度计读取。可以用3N HCl或3M H2SO4终止OPD反应，并在492 nm处读取。
应用
在酶联免疫吸附试验（ELISA）中，邻苯二胺盐酸盐用作辣根过氧化物酶偶联二抗的底物。
重悬
将 1 片片剂溶于 pH 值为 5.0的0.05M磷酸盐 - 柠檬酸盐缓冲液中至所需浓度（通常使用0.4mg / ml的OPD浓度）。临用前，向 100 mL 底物缓冲液中加入 40μl 新鲜过氧化氢（浓度为 30%）。片剂单独包装在铝箔包装中。
警示用语：
Warning
危险分类
Aquatic Chronic 2 - Carc. 2 - Muta. 2 - Skin Sens. 1
WGK
WGK 3
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商品
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硝基蓝四唑（NBT）可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐（BCIP）一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物，其颜色为蓝至紫色，可以通过视觉观察到。
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